Genistein-Supplemented Diet Decreases Malaria Liver Infection in Mice and Constitutes a Potential Prophylactic Strategy
Genistein-Supplemented Diet Decreases Malaria Liver Infection in Mice and Constitutes a Potential Prophylactic Strategy
复制标题
DOI：
10.1371/journal.pone.0002732
复制
发表时间：
2008-07-16
期刊：
影响因子：
3.7
通讯作者：
Mota, Maria M.
中科院分区：
文献类型：
--
作者：
Cunha-Rodrigues, Margarida;Portugal, Silvia;Mota, Maria M.
在热带地区，数百万人仍然生活在感染疟疾的危险之中。事实上，对氯喹和在这些地区使用的其他药物产生抗药性，加强了实施替代预防战略的必要性。染料木黄酮是一种天然存在的化合物，被广泛用作食品补充剂，并被认为是有效地对抗几种病理。这里介绍的结果表明，染料木黄酮抑制肝脏感染的疟原虫，疟疾的病原体。在体外，染料木黄酮通过抑制寄生虫细胞内发育的早期阶段，降低小鼠和人肝癌细胞的感染率。口服或腹腔注射金雀异黄素可降低伯氏疟原虫感染小鼠的肝脏寄生虫负荷。此外，喂食染料木黄酮补充饮食的小鼠显示出疟原虫肝脏感染的显著减少以及血液寄生虫血症的减少和对严重疾病的部分保护。由于染料木黄酮是一种安全，低成本，天然化合物，可以永久地用于饮食，我们建议将其用作地方性人群和长期旅行者的疟疾预防剂。
In tropical regions millions of people still live at risk of malaria infection. Indeed the emergence of resistance to chloroquine and other drugs in use in these areas reinforces the need to implement alternative prophylactic strategies. Genistein is a naturally occurring compound that is widely used as a food supplment and is thought to be effective in countering several pathologies. Results presented here show that genistein inhibits liver infection by the Plasmodium parasite, the causative agent of malaria. In vitro, genistein decreased the infection rates of both mouse and human hepatoma cells by inhibiting the early stages of the parasite's intracellular development. Oral or intraperitoneal administration of genistein decreased the liver parasite load of P. berghei-infected mice. Moreover, mice fed on a genistein-supplemented diet showed a significant reduction in Plasmodium liver infection as well as a reduced blood parasitemia and partial protection from severe disease. Since genistein is a safe, low-cost, natural compound that can be used permanently in a diet, we propose its use as a prophylactic agent against malaria for endemic populations and long-time travelers.