Functional differentiation and repertoire diversification of T cells derived from single progenitor cells
Functional differentiation and repertoire diversification of T cells derived from single progenitor cells
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源自单个祖细胞的 T 细胞的功能分化和谱系多样化
DOI：
10.1002/eji.1830180610
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发表时间：
1988
影响因子：
5.4
通讯作者：
Y. Katsura
中科院分区：
文献类型：
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作者：
T. Kina;T. Amagai;Shinsuke Nishikawa;Shin;Y. Katsura
研究了来源于单个祖细胞的T细胞的功能。B10.对Thy-1.1受体小鼠进行全身照射和骨髓重建，或胸腺屏蔽、照射和骨髓重建，并将有限数量（3 × 103或6 × 103）的C57 BL/6和B6.Lyt-2.1小鼠骨髓细胞1：1混合物胸腺内（i.t.）转移。供体类型一小部分受体的胸腺（Thy-1.2+）细胞表达Ly-2.2或Ly-2.1的表型，但不是两者都表达，被认为是来源于单个祖细胞的T细胞克隆，并测定这些克隆的多克隆辅助细胞（Th）和多克隆细胞溶解（CTL）活性以及同种异体抗原特异性增殖活性。（混合淋巴细胞反应; MLR）和CTL活性。在转移后4周采集的克隆（4周龄克隆）在全身照射受体的胸腺中产生，显示多克隆CTL但不显示多克隆Th活性，而在胸腺屏蔽受体的胸腺中产生的4周龄克隆显示多克隆CTL和Th活性。同样地，当在存在T细胞生长因子的情况下诱导时，在全身照射的受体中产生的4周龄克隆对同种异体抗原产生CTL应答，而不是MLR，而在胸腺屏蔽的受体中产生的4周龄克隆显示出对同种异体抗原的MLR和CTL。然而，4周龄克隆的谱系分化是不完整的，因为在全身照射的受体中产生的克隆不一定对检查的所有同种抗原产生CTL应答，而在胸腺屏蔽的受体中产生的克隆不一定对所有抗原产生MLR应答。另一方面，T细胞克隆显示在转移后7周在细胞功能以及库多样化方面完全成熟。
Functions of T cells derived from single progenitor cells were investigated. B10. Thy‐1.1 recipient mice were either whole body‐irradiated and marrow reconstituted or thymus‐shielded, irradiated and marrow reconstituted, and limited numbers (3 × 103 or 6 × 103) of a 1:1 mixture of bone marrow cells from C57BL/6 and B6.Lyt‐2.1 mice were transferred intrathymically (i.t.). Donor‐type (Thy‐1.2+) cells of the thymus of a small portion of recipients which expressed the phenotype of either Ly‐2.2 or Ly‐2.1 but not both were regarded to be a clone of T cells derived from a single progenitor cell, and such clones were assayed for polyclonal helper (Th) and polyclonal cytolytic (CTL) activities as well as alloantigen‐specific proliferative (mixed lymphocyte reaction; MLR) and CTL activities. Clones taken 4 weeks after transfer (4‐week‐old clones) which were generated in the thymus of whole body‐irradiated recipients showed polyclonal CTL but not polyclonal Th activity, whereas 4‐week‐old clones generated in the thymus of thymus‐shielded recipients showed both polyclonal CTL and Th activities. Similarly, 4‐week‐old clones generated in whole body‐irradiated recipients responded with CTL to alloantigens when induced in the presence of T cell growth factors but not with MLR, whereas 4‐week‐old clones generated in thymus‐shielded recipients showed both MLR and CTL to alloantigens. Repertoire difersifi‐cation of 4‐week‐old clones, however, was incomplete, since clones generated in whole body‐irradiated recipient did not necessarily respond with CTL to all alloantigens examined, and those generated in thymus‐shielded recipients did not necessarily respond with MLR to all the antigens. On the other hand, T cell clones were shown to fully mature by 7 weeks after transfer in terms of cell function as well as repertoire diversification.
T 细胞受体在体外早期胸腺细胞分化中的表达和作用。
DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Born,W;McDuffie,M;Roehm,N;Kushnir,E;White,J;Thorpe,D;Stefano,J;Kappler,J;Marrack,P
通讯作者：
Marrack,P