Therapeutic faecal microbiota transplantation controls intestinal inflammation through IL10 secretion by immune cells
Therapeutic faecal microbiota transplantation controls intestinal inflammation through IL10 secretion by immune cells
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DOI：
10.1038/s41467-018-07359-8
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发表时间：
2018-12-05
影响因子：
16.6
通讯作者：
Facciotti, Federica
中科院分区：
文献类型：
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作者：
Burrello, Claudia;Garavaglia, Federica;Facciotti, Federica
肠道微生物群的改变与不同的胃肠道疾病有关。通过粪便微生物群移植（FMT）恢复正常生物量被认为是一种有前途的治疗方法，即使其疗效的机制目前在很大程度上是未知的。在这里，我们试图阐明实验性结肠炎期间治疗性FMT给药对肠粘膜先天性和适应性免疫反应的功能影响。我们表明，治疗性FMT通过同时激活不同的免疫介导的途径减少结肠炎症并启动肠道稳态的恢复，最终导致先天性和适应性免疫细胞（包括CD 4（+）T细胞，iNKT细胞和抗原呈递细胞（APC））产生IL-10，并降低树突状细胞，单核细胞和巨噬细胞将MHCII依赖性细菌抗原呈递给结肠T细胞。这些结果证明了FMT治疗控制肠道实验性结肠炎的能力，并将FMT作为免疫相关病理学中有价值的治疗选择。
Alteration of the gut microbiota has been associated with different gastrointestinal disorders. Normobiosis restoration by faecal microbiota transplantation (FMT) is considered a promising therapeutic approach, even if the mechanisms underlying its efficacy are at present largely unknown. Here we sought to elucidate the functional effects of therapeutic FMT administration during experimental colitis on innate and adaptive immune responses in the intestinal mucosa. We show that therapeutic FMT reduces colonic inflammation and initiates the restoration of intestinal homeostasis through the simultaneous activation of different immune-mediated pathways, ultimately leading to IL-10 production by innate and adaptive immune cells, including CD4(+) T cells, iNKT cells and Antigen Presenting Cells (APC), and reduces the ability of dendritic cells, monocytes and macrophages to present MHCII-dependent bacterial antigens to colonic T cells. These results demonstrate the capability of FMT to therapeutically control intestinal experimental colitis and poses FMT as a valuable therapeutic option in immune-related pathologies.