Granules harboring translationally active mRNAs provide a platform for P-body formation following stress.
Granules harboring translationally active mRNAs provide a platform for P-body formation following stress.
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DOI：
10.1016/j.celrep.2014.09.040
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发表时间：
2014-11-06
期刊：
影响因子：
8.8
通讯作者：
Ashe MP
中科院分区：
文献类型：
--
作者：
Lui J;Castelli LM;Pizzinga M;Simpson CE;Hoyle NP;Bailey KL;Campbell SG;Ashe MP
mRNA定位于真核细胞中确定的胞质位点不仅允许定位的蛋白质产生，而且还决定mRNA的命运。例如，抑制性mRNA定位于P体和应激颗粒，它们的衰变和储存分别被引导。在这里，我们发现，几个mRNA定位于颗粒在无压力，积极生长的细胞。这些颗粒在P体的应力依赖性形成中起关键作用。特异性糖酵解mRNA共定位于每个细胞的多个颗粒中，其在P体形成期间聚集。在不形成P体的条件下或在突变体中仍然观察到这种聚集。在无应激的细胞中，mRNA颗粒似乎与主动翻译相关;这可能使来自相同途径或复合物的蛋白质表达的共调节成为可能。在这种协同调节和原核系统中操纵子的优势之间可以画出相似之处。特定的mRNA定位于活跃生长的细胞中的细胞质颗粒在应激后，颗粒充当P体形成的平台某些糖酵解mRNA共定位并在细胞质颗粒中翻译功能相关的mRNA的翻译可能在颗粒中共同调节。显示特定mRNA定位于活跃生长细胞中颗粒。这些mRNA颗粒与主动翻译相关，并被提议允许来自相同途径或复合物的蛋白质表达的共调节。在应力之后，颗粒聚集以驱动P体的形成。
The localization of mRNA to defined cytoplasmic sites in eukaryotic cells not only allows localized protein production but also determines the fate of mRNAs. For instance, translationally repressed mRNAs localize to P-bodies and stress granules where their decay and storage, respectively, are directed. Here, we find that several mRNAs are localized to granules in unstressed, actively growing cells. These granules play a key role in the stress-dependent formation of P-bodies. Specific glycolytic mRNAs are colocalized in multiple granules per cell, which aggregate during P-body formation. Such aggregation is still observed under conditions or in mutants where P-bodies do not form. In unstressed cells, the mRNA granules appear associated with active translation; this might enable a coregulation of protein expression from the same pathways or complexes. Parallels can be drawn between this coregulation and the advantage of operons in prokaryotic systems. Specific mRNAs are localized to cytoplasmic granules in actively growing cells After stress, the granules serve as a platform for P-body formation Certain glycolytic mRNAs colocalize and are translated in cytoplasmic granules Translation of functionally related mRNAs may be coregulated in the granules Lui et al. show that specific mRNAs localize to granules in actively growing cells. These mRNA granules are associated with active translation and are proposed to allow coregulation of protein expression from the same pathway or complex. Following stress, the granules aggregate to drive the formation of P-bodies.