Effect of leukocyte depletion on the function of the postobstructed kidney in the rat.
Effect of leukocyte depletion on the function of the postobstructed kidney in the rat.
复制标题
白细胞去除对大鼠梗阻后肾功能的影响。
DOI：
10.1038/ki.1989.181
复制
发表时间：
1989
影响因子：
19.6
通讯作者：
Klahr,S
中科院分区：
文献类型：
--
作者：
Harris,KP;Schreiner,GF;Klahr,S
白细胞消耗对大鼠梗阻后肾功能的影响。我们观察到输尿管梗阻后大量白细胞（主要是巨噬细胞）涌入肾皮质和髓质。为了研究这些浸润细胞对输尿管梗阻后GFR和RPF下降的潜在贡献，我们研究了16只雄性Lewis大鼠（体重246.4±4.0 g），在单侧释放24小时双侧输尿管梗阻（BUO）后3小时处于清醒状态。8只大鼠未接受辐射，8只大鼠在梗阻前一天接受1315拉德辐射。对另外8只大鼠输尿管梗阻24小时后的白细胞浸润进行了量化，并在事先照射和不照射的情况下进行了测量。照射减少皮质浸润（27.05±3.07 x 105vs）。1.2±0.83 × 105个细胞/g组织)和髓质浸润（13.6±1.79 × 105个细胞/g组织）。（0.86±0.45 × 105个细胞/g组织），阻塞后GFR增加（1.58±0.12比2.97±0.15 ml/min/kg体wt， P < 0.001）。11只大鼠，其中6只接受辐照，在不加BUO的情况下进行假手术，以评估单独辐照对肾功能的影响。照射对未梗阻大鼠肾功能无影响。BUO后尿中血栓素B2的排泄量增加，而BUO前的照射明显减弱了这种增加（9.53±2.14 pg/min vs. 32.46±4.95 pg/min vs. 19.03±1.94 pg/min）。辐照减少了钠和水的部分排泄。因此，浸润细胞的消除与梗阻后肾功能的改善有关，并表明浸润细胞可能通过产生血管活性前列腺素和其他尚未定义的产物来调节梗阻后肾功能的变化。
Effect of leukocyte depletion on the function of the postobstructed kidney in the rat. We have observed an influx of leukocytes, predominantly macrophages, into the cortex and medulla of the kidney following ureteral obstruction. To examine the potential contribution of these infiltrating cells to the decrease in GFR and RPF that occurs following ureteral obstruction, 16 male Lewis rats (wt 246.4 ± 4.0 g) were studied in the awake state three hours after unilateral release of 24 hours of bilateral ureteral obstruction (BUO). Eight rats were not irradiated, and eight rats received 1315 rads one day prior to the obstruction. The leukocyte infiltrate following 24 hours of ureteral obstruction was quantified with and without prior irradiation in an additional eight rats. Irradiation reduced cortical infiltration (27.05 ± 3.07 x 105vs. 1.2 ± 0.83 x 105cells/g tissue) and medullary infiltration (13.6 ± 1.79 x 105vs. 0.86 ± 0.45 x 105cells/g tissue) of leukocytes following BUO (P < 0.001 for both) and increased postobstruction GFR (1.58 ± 0.12 vs. 2.97 ± 0.15 ml/min/kg body wt, P < 0.001). Eleven rats, six of which received irradiation, underwent sham laparotomy without BUO in order to assess the effect of irradiation alone on renal function. Irradiation had no effect on the renal function of non-obstructed rats. Urinary excretion of thromboxane B2 increased following BUO and this rise was significantly blunted by irradiation prior to BUO (9.53 ± 2.14 vs. 32.46 ± 4.95 vs. 19.03 ± 1.94 pg/min). Fractional excretion of sodium and water was reduced by irradiation. Thus, abolition of infiltrating cells is associated with an improvement in postobstructive renal function and suggests that infiltrating cells may modulate the changes in renal function following obstruction, possibly via the production of vasoactive prostanoids and other as yet undefined products.