Factors influencing the adherence of Pseudomonas aeruginosa to mammalian buccal epithelial cells.
Factors influencing the adherence of Pseudomonas aeruginosa to mammalian buccal epithelial cells.
复制标题
影响铜绿假单胞菌与哺乳动物颊上皮细胞粘附的因素。
DOI：
10.1093/clinids/5.supplement_5.s846
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Bass,JA
中科院分区：
文献类型：
--
作者：
Woods,DE;Straus,DC;JohansonJr,WG;Bass,JA
先前已经证明了重症患者上呼吸道上皮细胞体外粘附铜绿假单胞菌与该机会致病菌随后在呼吸道定植之间的相关性。虽然在体内细胞表面的特定改变，允许粘附的P。aerodyna尚未定义，我们已经证明，P。体外嗜氧菌粘附可能与细胞表面蛋白酶敏感性糖蛋白纤连蛋白的丢失有关。这些研究的目的是在体外粘附的P。本发明的目的是将来自患者的aerodynosato颊上皮细胞与来自相同患者的细胞表面纤连蛋白和唾液蛋白酶的水平进行比较，以及确定在粘附过程中重要的细菌表面上的结构。一个直接的放射免疫结合试验被开发来测量细胞表面的纤维连接蛋白，而分泌物中的蛋白酶活性通过125 I-标记的不溶性纤维蛋白基质的125 I释放来测量。放射性标记P.嗜酸性粒细胞增多症与细胞表面纤维连接蛋白减少（P<0.001）和唾液蛋白酶水平增加（P<0.001）直接相关。此外，我们还证实了皮利可能介导了P. aerodynosato颊细胞。当与口腔细胞一起预孵育时，纯化的皮利的能力表明了这一点，将完整生物体的粘附从平均30.6个生物体/细胞降低到5.7个生物体/细胞（P<0.01）。
A correlation has previously been demonstrated between the in vitro adherence ofPseudomonas aeruginosato upper respiratory tract epithelium of seriously ill patients and subsequent colonization of the respiratory tract by this opportunistic pathogen. Although the specific in vivo alterations in the cell surface that permit the adherence ofP. aeruginosahave not been defined, we have demonstrated thatP. aeruginosaadherence in vitro can be correlated with the loss of a protease-sensitive glycoprotein, fibronectin, from the cell surface. The object of these studies was to correlate in vitro adherence ofP. aeruginosato buccal epithelial cells from patients with the levels of cell-surface fibronectin and of salivary proteases from the same patients, as well as to define the structure(s) on the bacterial surface important in the adherence process. A direct radioimmuno-binding assay was developed to measure cell-surface fibronectin, while protease activity in secretions was measured by125I release from125I-labelled insoluble fibrin matrices. Adherence of radiolabeledP. aeruginosawas directly related to decreased amounts of cell-surface fibronectin (P< .001) and increased levels of salivary protease (P< .001). Additionally, we demonstrated that pili may mediate the adherence ofP. aeruginosato buccal cells. This was shown by the ability of purified pili, when preincubated with buccal cells, to decrease the adherence of intact organisms from a mean of 30.6 organisms/cell to 5.7 organisms/cell (P< .01).