REQUIREMENT OF INNERVATION FOR MAINTENANCE OF STRUCTURAL AND FUNCTIONAL INTEGRITY IN THE RAT PROSTATE
REQUIREMENT OF INNERVATION FOR MAINTENANCE OF STRUCTURAL AND FUNCTIONAL INTEGRITY IN THE RAT PROSTATE
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DOI：
10.1095/biolreprod44.6.1171
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发表时间：
1991-06-01
影响因子：
3.6
通讯作者：
LEE, C
中科院分区：
文献类型：
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作者：
WANG, JM;MCKENNA, KE;LEE, C
大鼠前列腺由盆腔神经和腹下神经通过单个盆神经节支配，切除盆神经节将导致前列腺复合体完全失神经。本研究旨在探讨去神经对大鼠前列腺的影响。成年SD大鼠，体重300g，随机分为两组。其中一组动物行双侧盆神经节去神经术，另一组动物行假手术。三周后，动物被处死；切除前列腺腹侧，称其重量，并接受常规的光镜和电子显微镜检查。失神经组大鼠前列腺腹侧的平均重量、总蛋白含量和酸性磷酸酶总含量均显著低于对照组。然而，每一前列腺的总DNA含量在两组之间没有显著差异。组织学检查结果显示，对照动物的前列腺形态正常，而去神经动物的前列腺细胞高度降低，细胞核周围空泡增大。电子显微镜检查显示，与对照组相比，去神经动物的前列腺细胞高度与细胞宽度的比率降低，这与微绒毛和分泌颗粒的数量减少有关。这些结果表明，前列腺失神经与前列腺重量降低、细胞高度降低和分泌活动减少有关。
The rat prostate is innervated by pelvic and hypogastric nerves through a single pelvic ganglion, removal of which would result in complete denervation of the prostatic complex. The present study was conducted to investigate the effect of denervation on the rat prostate. Adult Sprague-Dawley rats of 300 g in body weight were divided into two groups. Bilateral pelvic ganglion denervation was performed in one group of animals; the other group received a sham operation. Three weeks later, animals were killed; and the ventral prostates were removed, weighed, and subjected to routine procedures for light and electron microscopy. The average weight, total protein content, and total content of acid phosphatase of the ventral prostates in the denervated group were significantly less than those in the control group. Total DNA content per prostate, however, was not significantly different between the two groups. Results of histological examination indicated that the prostates from control animals showed normal morphology, whereas those from the denervated animals showed reduced cell height and enlarged vacuoles surrounding the nuclei. Inspection of electron micrographs revealed that the prostates from denervated animals, compared with those of controls, showed a reduced ratio of cell height:cell width, which is associated with a reduced number of microvilli and secretory granules. These results indicate that denervation in the prostate is associated with a lower prostatic weight, decreased cell height, and reduced secretory activity.