Dual-antibody Modified PLGA Nanofibers for Specific Capture of Epithelial and Mesenchymal CTCs
Dual-antibody Modified PLGA Nanofibers for Specific Capture of Epithelial and Mesenchymal CTCs
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用于特异性捕获上皮和间质 CTC 的双抗体修饰 PLGA 纳米纤维
DOI：
10.1016/j.colsurfb.2019.05.031
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发表时间：
2019-09-01
影响因子：
5.8
通讯作者：
Pei, Renjun
中科院分区：
文献类型：
--
作者：
Liu, Hui;Wang, Zhili;Pei, Renjun
CTC检测在癌症早期诊断、患者预后、个体化治疗、肿瘤进展监测等方面具有重要的临床意义。研究表明，疾病进展与间充质CTC的增加有关，而目前成熟的检测技术主要是基于上皮细胞CTC的检测。在目前的工作中，开发了针对EpCAM和N-Cadherin的双抗体纳米界面，以从血液样品中捕获上皮CTC以及间充质CTC。通过静电纺丝制造均匀的聚-（乳酸共-乙醇酸）（PLGA）微球基底以提供用于细胞捕获的平台（即，MCF-7和GIST 882细胞），用BSA和双重抗体修饰。我们的结果显示，双抗体底物相比于单抗体底物表现出提高的靶细胞捕获效率，揭示了双抗体PLGA纳米纤维在临床上用于有效捕获上皮和间充质CTC的潜在应用。
CTC detection has great potential to provide crucial clinical information for early cancer diagnosis, patient prognosis, personalized therapy, and cancer progress monitoring etc. It has been proved that the disease progress is associated with an increase in mesenchymal CTCs, while most mature techniques have been developed based on epithelial CTC detection. In present work, a dual-antibody nanointerface against EpCAM and N-Cadherin was developed to capture epithelial CTCs as well as mesenchymal CTCs from blood samples. A uniform poly-(lactic co-glycolic acid) (PLGA) nanofiber substrate was fabricated by electrospinning to provide a platform for cell capture (i.e., MCF-7 and GIST882 cells), modified with BSA and dual antibodies. Our results showed the dual antibody substrates exhibited an improved capture efficiency of target cells compared to the mono-antibody ones, revealing potential application of the dual-antibody PLGA nanofibers for efficient capture of epithelial and mesenchymal CTCs in clinic.