Plasma 3-nitrotyrosine is elevated in premature infants who develop bronchopulmonary dysplasia
Plasma 3-nitrotyrosine is elevated in premature infants who develop bronchopulmonary dysplasia
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DOI：
10.1542/peds.101.5.870
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发表时间：
1998-05-01
期刊：
影响因子：
8
通讯作者：
Ballard, RA
中科院分区：
文献类型：
--
作者：
Banks, BA;Ischiropoulos, H;Ballard, RA
Objective.早产儿易患支气管肺发育不良（BPD），这是一种婴儿期慢性肺部疾病，部分原因是高氧引起的氧化应激。为了研究一氧化氮衍生的氧化剂如过氧亚硝酸盐在BPD病因学中的可能作用，我们测量了血浆3-硝基酪氨酸的水平，其由过氧亚硝酸盐与蛋白质的反应产生。从一组参加产前治疗临床试验的受试者中回顾性地确定了10例发生BPD的早产儿，定义为超过月经后36周需要补充氧气。作为试验的一部分，在这些婴儿出生后的第一个月内收集了一系列血浆样本。16例早产儿来自同一人群：5例无肺部疾病，6例呼吸窘迫综合征消退，5例在出生后28天有残留肺部疾病，经后36周消退。采用固相免疫放射化学法测定血浆3-硝基酪氨酸水平。无BPD婴儿的所有3-硝基酪氨酸值均为
Objective. Premature infants are susceptible to bronchopulmonary dysplasia (BPD), a chronic lung disease of infancy that appears to be caused in part by oxidative stress from hyperoxia. To investigate the possible role of nitric oxide-derived oxidants such as peroxynitrite in the etiology of BPD, we measured levels of plasma 3-nitrotyrosine, which is produced by the reaction of peroxynitrite with proteins.Patients and Methods. Ten premature infants who developed BPD, defined as requiring supplemental oxygen beyond 36 weeks' postmenstrual age, were identified retrospectively from a group of subjects enrolled in a clinical trial of antenatal therapy. Serial plasma samples had been collected on these infants during the first month of life as part of the trial. Sixteen comparison premature infants were identified from the same population: 5 had no lung disease, 6 had respiratory distress syndrome that resolved, and 5 had residual lung disease at 28 days of life that resolved by 36 weeks' postmenstrual age. Plasma 3-nitrotyrosine levels were measured using a solid phase immunoradiochemical method.Results. All 3-nitrotyrosine values in infants without BPD were