NMDA and purinergic processes modulate neck muscle activity evoked by noxious stimulation of dura
NMDA and purinergic processes modulate neck muscle activity evoked by noxious stimulation of dura
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NMDA 和嘌呤能过程调节硬脑膜有害刺激引起的颈部肌肉活动
DOI：
10.1111/jop.13072
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发表时间：
2020-07-01
影响因子：
3.3
通讯作者：
Sessle，Barry J.
中科院分区：
文献类型：
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作者：
Yao，Dongyuan;Li，Jian;Sessle，Barry J.
背景三磷酸腺苷（ATP）和谷氨酸与一些头痛症状有关，髓质中的嘌呤能（P2X）和谷氨酸N-甲基-D-天冬氨酸（NMDA）受体相关过程可以调节进入脑干的三叉神经伤害性传入输入对颅面感觉运动回路的影响。本研究旨在测试是否可以通过涉及 P2X 或 NMDA 受体依赖性机制的额部硬脑膜或上矢状窦的有害刺激来诱导大鼠颈部肌肉活动。方法在麻醉大鼠 (n = 46) 中记录颈部和颅面肌肉的肌电活动时，将炎性刺激性芥子油（0.2 µL，20% MO）或媒介物（矿物油）局部应用于硬脑膜或窦，之前加入 10 µL ATP 拮抗剂 2',3'-O-(2,4,6- 三硝基苯基) 腺苷 5'-三磷酸 (TNP-ATP，0.1 mmol/L；n = 8) 或 2-氨基-5-膦基戊酸 (APV，0.05 mmol/L；n = 7) 或磷酸盐缓冲盐水（PBS 作为载体）对照；n = 10)。结果将 MO 而不是媒介物应用于额叶硬脑膜显着增加 (P<.05) 颈部肌电活动，而将 MO 应用于上矢状窦不会显着增加颈部肌电活动，除非 MO 先前已应用于硬脑膜。使用 TNP-ATP 或 APV 进行预处理 (i.t.)，但不使用媒介物对照，显着降低了 MO 应用于硬脑膜引起的颈部肌电活动。结论这些数据表明，额叶硬脑膜（但不是上矢状窦）的有害刺激可能会增强 P2X 和 NMDA 受体依赖性的颈部肌肉活动。这些影响可能会导致某些头痛情况下出现的颈部肌肉僵硬。
BackgroundAdenosine triphosphate (ATP) and glutamate are associated with some headache conditions, and purinergic (P2X) and glutamatergicN‐methyl‐D‐aspartate (NMDA) receptor‐related processes in the medulla can modulate the effects of trigeminal nociceptive afferent inputs into the brainstem on craniofacial sensorimotor circuits. This study aimed to test whether neck muscle activity can be induced in rats by noxious stimulation of the frontal dura or superior sagittal sinus that involves P2X or NMDA receptor‐dependent mechanisms.MethodsWhile electromyographic activities of neck and craniofacial muscles were being recorded in anesthetized rats (n = 46), the inflammatory irritant mustard oil (0.2 µL, 20% MO) or vehicle (mineral oil) was topically applied to the dura or sinus, preceded by 10 µL of the ATP antagonist 2′,3′‐O‐(2,4,6‐ trinitrophenyl) adenosine 5′‐triphosphate (TNP‐ATP, 0.1 mmol/L; n = 8) or 2‐amino‐5‐phosphonopentanoic acid (APV, 0.05 mmol/L; n = 7) or phosphate‐buffered saline (PBS as vehicle control; n = 10).ResultsApplication of MO but not vehicle to the frontal dura significantly increased (P< .05) neck electromyographic activity whereas MO application to the superior sagittal sinus did not significantly increase neck electromyographic activity unless MO had previously been applied to the dura. Pre‐treatment (i.t.) with TNP‐ATP or APV but not vehicle control significantly reduced neck electromyographic activity evoked by MO application to the dura.ConclusionsThese data suggest that noxious stimulation of the frontal dura (but not superior sagittal sinus) may enhance neck muscle activity that is P2X and NMDA receptor‐dependent. These effects may contribute to neck muscle stiffness that occurs in some headache conditions.