Developmental expression of the homeobox protein Distal-less 3 and its relationship to progesterone production in mouse placenta
Developmental expression of the homeobox protein Distal-less 3 and its relationship to progesterone production in mouse placenta
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DOI：
10.1677/joe.1.06217
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发表时间：
2005-08-01
影响因子：
4
通讯作者：
Roberson, MS
中科院分区：
文献类型：
--
作者：
Berghorn, KA;Clark, PA;Roberson, MS
远端缺失3 （Dlx3）是一种同源盒因子，作为胎盘特异性转录调节因子。Dlx3缺失小鼠（-/-）胎盘发育受损，不能在子宫内存活超过胚胎日(E) 9-5。目前的研究旨在检测Dlx3在妊娠期小鼠胎盘中的表达，并确定Dlx3是否参与胎盘黄体酮的产生。Dlx3在E8.5未检测到，但在E9.5胎盘中检测到，并在E15.5胎盘中持续表达，但表达减少。Dlx3免疫定位局限于迷宫，是核的，在细胞角蛋白阳性的细胞中发现。之前对绒毛膜癌细胞系的研究支持这样的结论：3 β -羟基类固醇脱氢酶VI （3 β HSD VI）的表达需要Dlx3, 3 β HSD VI是滋养层巨细胞产生孕酮的专性酶。在大鼠滋养细胞干细胞系（rcho - 1）中，使用氮源诱导分化的rcho - 1细胞中检测不到Dlx3的表达。Dlx3（+/+）、（+/ -）和（- / -）小鼠胎盘培养物和3 β HSD VI rriRNA的体外孕酮产量相等。3 β HSD VI的原位杂交显示mRNA的表达仅限于滋养层巨细胞，而迷宫中未检测到mRNA的表达，这表明Dlx3和3 β HSD VI在胎盘内没有共定位。这些研究支持了Dlx3蛋白表达仅限于妊娠晚期小鼠胎盘迷宫区，并且Dlx3在滋养层巨细胞中似乎不表达的结论。此外，Dlx3的缺失与E9.5小鼠胎盘中黄体酮的合成无关。
Distal-less 3 (Dlx3) is a homeobox factor that functions as a placental-specific transcriptional regulator. Dlx3 null mice (-/-) have compromised placental development and do not survive in utero past embryonic day (E) 9-5. The current studies were undertaken to examine the expression of Dlx3 in mouse placenta during gestation, and to determine whether Dlx3 was involved in placental progesterone production. Dlx3 was not detectable at E8.5 but was detected in E9.5 placenta with continuing but diminished expression through E15.5. Dlx3 immunolocalization was restricted to the labyrinth, was nuclear and was found in cytokeratin-positive cells. Previous studies in choriocarcinoma cell lines support the conclusion that Dlx3 is required for expression of 3 beta-hydroxysteroid dehydrogenase VI (3 beta HSD VI), an obligate enzyme in the production of progesterone by trophoblast giant cells. In a rat trophoblast stem cell line (Rcho-l), Dlx3 expression was non-detectable in Rcho-l cells induced to differentiate using mitrogen withdrawal. In vitro progesterone production in placental cultures and 3 beta HSD VI rriRNA from Dlx3 (+/+), (+/ -) and (- / -) mice were equivalent. In situ hybridization for 3 beta HSD VI revealed mRNA expression restricted to trophoblast giants cells with no detectable expression in the labyrinth suggesting that Dlx3 and 3 beta HSD VI were not colocalized within the placenta. These studies support the conclusion that Dlx3 protein expression is restricted to the labyrinth region of the murine placenta into late gestation and that Dlx3 does not appear to be expressed in trophoblast giant cells. Further, loss of Dlx3 was not correlated with synthesis of progesterone from E9.5 mouse placentas.