Bridging small-gap peripheral nerve defects using acellular nerve allograft implanted with autologous bone marrow stromal cells in primates
Bridging small-gap peripheral nerve defects using acellular nerve allograft implanted with autologous bone marrow stromal cells in primates
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DOI：
10.1016/j.brainres.2007.09.098
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发表时间：
2008-01-10
期刊：
影响因子：
2.9
通讯作者：
Yi, Jian-Hua
中科院分区：
文献类型：
--
作者：
Wang, Dong;Liu, Xiao-Lin;Yi, Jian-Hua
本研究评价了组织工程神经移植的效果，来自脱细胞异体神经移植物，结合自体骨髓基质细胞（MSCs），到周围神经缺损。在恒河猴模型中，通过使用注射有自体MSC的脱细胞同种异体神经，在桡神经中的Icm损伤处评估神经再生。单纯脱细胞同种异体神经移植作为对照。术后8周，进行免疫荧光染色、组织形态学分析和电生理评价。荧光显微镜显示，一些MSC免疫阳性S-100蛋白，表明雪旺细胞（SC）的表型。用培养的MSC治疗的组显示出统计学上更高数量的神经纤维，具有形状良好的有髓鞘轴突。骨髓间充质干细胞组运动神经传导速度和复合肌肉动作电位（CMAP）峰值均高于对照组。这一结果表明，骨髓间充质干细胞能够在体内分化为施万样细胞，并促进灵长类动物的神经再生。此外，与单独的脱细胞神经相比，注射MSC的脱细胞神经为再生轴突的生长和髓鞘形成提供了有利的环境。(c)2007 Elsevier B. V.保留所有权利。
This study evaluated the effects of the transplantation of a tissue-engineered nerve derived from an acellular allogenic nerve graft, combined with autologous bone marrow stromal cells (MSCs), into peripheral nerve defects. In a rhesus monkey model, nerve regeneration was evaluated across a 1-cm lesion in the radial nerve by using an acellular allogenic nerve injected with autologous MSCs. Simple acellular nerve allografts served as control. Eight weeks after surgery, immunofluorescence staining, histologic morphometrical analysis and electrophysiologic evaluation were performed. Fluorescence microscopy revealed that some MSCs were immunopositive to S-100 protein, indicating a Schwann cell (SC) phenotype. The group treated with cultured MSCs showed a statistically higher number of nerve fibers, with well-shaped remyelinated axons. The motor conduction velocities and the peak amplitudes of compound muscle action potentials (CMAP) for the group treated with MSCs were higher than those of the controls. This outcome indicated that MSCs are able to differentiate into Schwann-like cells in vivo and to promote nerve regeneration in primates. Furthermore, the acellular nerves injected with MSCs provided a favorable environment for the growth and myelination of regenerating axons when compared to acellular nerves alone. (c) 2007 Elsevier B.V. All rights reserved.