Effect of 2′-fucosyllactose supplementation on intestinal flora in mice with intestinal inflammatory diseases
Effect of 2′-fucosyllactose supplementation on intestinal flora in mice with intestinal inflammatory diseases
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DOI：
10.1016/j.idairyj.2020.104797
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发表时间：
2020-11-01
影响因子：
3.1
通讯作者：
Liu, Li-bo
中科院分区：
文献类型：
--
作者：
Li, Ai-li;Ni, Wei-wei;Liu, Li-bo
评价了人乳低聚糖（HMO）及其主要成分2 '-岩藻糖基乳糖（2'-FL）对肠道疾病的影响。建立葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠模型，以研究HMO和2 '-FL在减轻结肠炎和调节肠道微生物群中的相关性。结果表明，食用HMOs和2 '-FL均能减轻炎症性肠病。此外，膳食HMOs和2'-FL均能抑制链球菌和土氏志贺菌的过度生长。HMO增加了胞壁杆菌科和拟杆菌的比例，2 '-FL显著增加了乳酸杆菌的丰度。这与短链脂肪酸的产生呈正相关，但与总体炎症生物标志物呈负相关。PIC-RUSt分析进一步表明，HMO和2 '-FL都调节与感染性疾病和碳水化合物代谢相关的细菌基因。我们的研究结果表明，2 '-FL具有类似于HMO的抗炎活性，这可能与调节肠道植物群和代谢产物有关。(C)2020爱思唯尔有限公司保留所有权利。
The effects of human milk oligosaccharides (HMOs) and the main component, 2'-fucosyllactose (2'-FL), on intestinal disorders were evaluated. A dextran sodium sulphate (DSS)-induced mice model for colitis was established to investigate the correlation between HMOs and 2'-FL in attenuating colitis and modulating gut microbiota. The results indicated that inflammatory bowel disease was attenuated by consumption of both HMOs and 2'-FL. Moreover, both dietary HMOs and 2'-FL inhibited the overgrowth of Streptococcus and Escherichia-Shigella. HMOs increased the proportion of Muribaculaceae and Bacteroides, and 2'-FL dramatically increased Lactobacillus abundance. This was positively correlated with short chain fatty acid production, but was negatively correlated with inflammatory biomarkers overall. PIC-RUSt analysis further showed that both HMO and 2'-FL regulated the bacterial genes related to infectious diseases and carbohydrate metabolism. Our findings suggested that 2'-FL had anti-inflammatory activities similar to those of HMOs; this might be related to regulation of the intestinal flora and metabolites. (C) 2020 Elsevier Ltd. All rights reserved.