Isolation and characterization of highly and rarely metastatic clones from murine colon adenocarcinoma 26.
Isolation and characterization of highly and rarely metastatic clones from murine colon adenocarcinoma 26.
复制标题
鼠结肠腺癌高度和罕见转移性克隆的分离和表征26。
DOI：
--
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Y. Sakurai
中科院分区：
文献类型：
--
作者：
T. Yamori;T. Tsuruo;K. Naganuma;S. Tsukagoshi;Y. Sakurai
从小鼠结肠腺癌26细胞系(Colon 26)体外分离到三个典型的转移克隆，分别命名为N-1、N-4和N-5。自然转移率以N-4最高(85%)，N-5和26结肠中等(分别为50%和53%)，N-1最低(0%)。转移的主要靶器官是肺。在这些克隆中，N-4在静脉接种后具有较高的肺定植能力、较高的成瘤性和较高的培养饱和密度。用125I标记的凝集素对克隆细胞的细胞表面分析显示，在高转移的N-4细胞中，刀豆蛋白A(ConA)结合位点的数量显著减少。在细胞糖蛋白的十二烷基硫酸钠平板凝胶分析中，与其他克隆和亲本结肠26相比，N-1中与ConA反应的94,000-Dalton组分更强烈。这些克隆可为结肠癌转移的研究提供新的模型。
Three typical metastatic clones, designated N-1, N-4, and N-5, were isolated in vitro from a murine colon adenocarcinoma 26 cell line (Colon 26). The incidence of spontaneous metastasis was highest in N-4 (85%), moderate in N-5 and Colon 26 (50 and 53%, respectively) and lowest in N-1 (0%). The major target organ of metastasis was the lung. Among the clones, N-4 showed higher lung colonizing potential after intravenous inoculation, higher tumorigenicity and higher saturation density in culture. Cell-surface analysis of cloned cells by 125I-labeled lectins revealed significant reduction of the number of concanavalin A (Con A)-binding sites in highly metastatic N-4 cells. In sodium dodecyl sulfate-slab gel analysis of cellular glycoproteins, a 94,000-dalton component, which is reactive to Con A, was more intensely observed in N-1 as compared with other clones and parental Colon 26. These clones could provide a new model for the study of metastasis of colon carcinoma.