Metabonomic studies on potential plasma biomarkers in rats exposed to ionizing radiation and the protective effects of Hong Shan Capsule
Metabonomic studies on potential plasma biomarkers in rats exposed to ionizing radiation and the protective effects of Hong Shan Capsule
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DOI：
10.1007/s11306-013-0529-6
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发表时间：
2013-10-01
期刊：
影响因子：
3.6
通讯作者：
Chai, Yi-Feng
中科院分区：
文献类型：
--
作者：
Liu, Yue;Lin, Ze-Bin;Chai, Yi-Feng
基于超高效液相色谱-质谱联用的代谢组学方法，结合PCA、PLS-DA、RF和热图等模式识别方法，研究了与电离辐射（IR）相关的全球血清代谢谱。由于代谢物的vip值阈值被设置为2，高于该阈值的代谢物作为潜在的目标生物标志物被过滤掉。在大鼠血浆中鉴定出19种不同的潜在生物标志物。为了进一步阐明IR的病理生理机制，研究了相关的代谢途径。发现IR与紊乱脂肪酸代谢、牛磺酸和次牛磺酸代谢、鞘脂代谢、嘌呤代谢、嘧啶代谢、磷脂分解代谢、色氨酸代谢、苯丙氨酸代谢、胆酸代谢密切相关。采用代谢组学方法，系统分析了中药红山胶囊（HSC）的保护作用。结果表明，HSC可以通过部分调节紊乱的代谢途径对IR产生满意的影响。
An ultra performance liquid chromatography coupled to mass spectrometry-based metabonomic approach, combined with pattern recognition methods including PCA, PLS-DA, RF and heatmap, has been developed to characterize the global serum metabolic profile associated with ionizing radiation (IR). As the VIP-value threshold cutoff of the metabolites was set to 2, metabolites above this threshold were filtered out as potential target biomarkers. Nineteen distinct potential biomarkers in rat plasma were identified. To further elucidate the pathophysiology of IR, related metabolic pathways have been studied. It was found that IR was closely related to disturbed fatty acid metabolism, taurine and hypotaurine metabolism, sphingolipid metabolism, purine metabolism, pyrimidine metabolism, phospholipid catabolism, tryptophan metabolism, phenylalanine metabolism, and bile acid metabolism. With the presented metabonomic method, we systematically analyzed the protective effects of Traditional Chinese Medicine Hong Shan Capsule (HSC). The results demonstrated that HSC administration could provide satisfactory effects on IR through partially regulating the perturbed metabolic pathways.