A time-course Raman spectroscopic analysis of spontaneous in vitro microcalcifications in a breast cancer cell line.
A time-course Raman spectroscopic analysis of spontaneous in vitro microcalcifications in a breast cancer cell line.
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乳腺癌细胞系体外自发微钙化的时程拉曼光谱分析。
DOI：
10.1038/s41374-021-00619-0
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发表时间：
2021-09
期刊：
影响因子：
--
通讯作者：
Stone N
中科院分区：
文献类型：
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作者：
Bouzy P;O'Grady S;Madupalli H;Tecklenburg M;Rogers K;Palombo F;Morgan MP;Stone N
微钙化是乳腺癌的早期标志物，可以提供有价值的预后信息，以支持临床决策。目前对乳腺组织中钙化的检测是基于X射线乳房摄影术，其涉及使用具有潜在有害影响的电离辐射，或MRI扫描，其具有有限的空间分辨率。此外，这些技术不能区分微钙化与良性和恶性病变。几项研究表明，振动光谱技术能够区分和分类乳腺病变，病理分级的基础上的化学成分的微钙化。然而，乳腺微钙化的发生和潜在的矿化过程仍然没有完全了解。使用先前建立的体外矿化模型，使用两种成骨剂无机磷酸盐（Pi）和β-甘油磷酸盐（βG）诱导MDA-MB-231人乳腺癌细胞系，并使用拉曼显微光谱法直接监测矿化过程。在补充有Pi的培养基中培养的MDA-MB-231细胞比暴露于βG的细胞（到第11天）呈现更快的矿化（到第3天）。补充βG的细胞的磷酸盐伸缩峰的红移揭示了在磷灰石晶体形成期间存在不同的前体相（磷酸八钙）。这些结果表明，拉曼显微光谱是矿物物种无损分析的有力工具，如果用于病理样本，可以为评估矿化动力学和任何相关的乳腺癌进展提供有价值的信息。利用拉曼显微光谱研究了MDA-MB-231乳腺细胞的矿化。无机磷酸盐（Pi）和β-甘油磷酸盐（β-Glycerophosphate，βG）诱导矿化。结果表明，在羟基磷灰石晶体的形成过程中，Pi的吸收使矿化速度加快，βG的吸收表明存在前驱相。
Microcalcifications are early markers of breast cancer and can provide valuable prognostic information to support clinical decision-making. Current detection of calcifications in breast tissue is based on X-ray mammography, which involves the use of ionizing radiation with potentially detrimental effects, or MRI scans, which have limited spatial resolution. Additionally, these techniques are not capable of discriminating between microcalcifications from benign and malignant lesions. Several studies show that vibrational spectroscopic techniques are capable of discriminating and classifying breast lesions, with a pathology grade based on the chemical composition of the microcalcifications. However, the occurrence of microcalcifications in the breast and the underlying mineralization process are still not fully understood. Using a previously established model of in vitro mineralization, the MDA-MB-231 human breast cancer cell line was induced using two osteogenic agents, inorganic phosphate (Pi) and β-glycerophosphate (βG), and direct monitoring of the mineralization process was conducted using Raman micro-spectroscopy. MDA-MB-231 cells cultured in a medium supplemented with Pi presented more rapid mineralization (by day 3) than cells exposed to βG (by day 11). A redshift of the phosphate stretching peak for cells supplemented with βG revealed the presence of different precursor phases (octacalcium phosphate) during apatite crystal formation. These results demonstrate that Raman micro-spectroscopy is a powerful tool for nondestructive analysis of mineral species and can provide valuable information for evaluating mineralization dynamics and any associated breast cancer progression, if utilized in pathological samples. The mineralization of MDA-MB-231 breast cells was investigated using Raman micro-spectroscopy. Mineralization was induced by two osteogenic agents: inorganic phosphate (Pi) and β-Glycerophosphate (βG). The results show that the uptake of Pi allows a faster mineralization and the uptake of βG indicated the presence of a precursor phase during the hydroxyapatite crystal formation.