Enhanced Bacterial Growth by Polyelemental Glycerolate Particles
Enhanced Bacterial Growth by Polyelemental Glycerolate Particles
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DOI：
10.1021/acsabm.2c01052
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发表时间：
2023-03-18
影响因子：
4.7
通讯作者：
Shahbazian-Yassar，Reza
中科院分区：
文献类型：
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作者：
Phakatkar，Abhijit H.;Goncalves，Josue M.;Shahbazian-Yassar，Reza
虽然多元素合金在医疗保健应用方面很有前景，但它们在促进细菌生长方面的有效性仍未得到探索。在本工作中，我们评估了多元素甘油颗粒（PGPs）与大肠杆菌(E。杆菌)的细菌。采用溶剂热法合成了聚丙烯酸甲酯，证实了聚丙烯酸甲酯的甘油基质中金属阳离子的纳米尺度随机分布。我们观察到e增长了7倍。与对照le相比，大肠杆菌与五酰甘油（NiZnMnMgSr-Gly）颗粒相互作用4小时。colibacteria。细菌与pgp相互作用的纳米尺度微观研究表明，pgp释放了细菌细胞质中的金属阳离子。电镜成像和化学图谱显示细菌在pgp上形成生物膜，但没有造成明显的细胞膜损伤。数据表明，甘油在pgp中的存在可以有效地控制金属阳离子的释放，从而防止细菌毒性。多种金属阳离子的存在有望提供细菌生长所需的营养物质的协同效应。目前的工作为pgp促进生物膜生长的机制提供了关键的微观见解。这项研究为pgp在细菌生长至关重要的领域（包括医疗保健、清洁能源和食品工业）的未来应用打开了大门。
While polyelemental alloys are shown to be promising for healthcare applications, their effectiveness in promoting bacterial growth remains unexplored. In the present work, we evaluated the interaction of polyelemental glycerolate particles (PGPs) withEscherichia coli(E. coli) bacteria. PGPs were synthesized using the solvothermal route, and nanoscale random distribution of metal cations in the glycerol matrix of PGPs was confirmed. We observed 7-fold growth ofE. colibacteria upon 4 h of interaction with quinary glycerolate (NiZnMnMgSr-Gly) particles in comparison to controlE. colibacteria. Nanoscale microscopic studies on bacteria interactions with PGPs showed the release of metal cations in the bacterium cytoplasm from PGPs. The electron microscopy imaging and chemical mapping indicated bacterial biofilm formation on PGPs without causing significant cell membrane damage. The data showed that the presence of glycerol in PGPs is effective in controlling the release of metal cations, thus preventing bacterial toxicity. The presence of multiple metal cations is expected to provide synergistic effects of nutrients needed for bacterial growth. The present work provides key microscopic insights of mechanisms by which PGPs enhance biofilm growth. This study opens the door for future applications of PGPs in areas where bacterial growth is essential including healthcare, clean energy, and the food industry.