Runx3-/- gastric epithelial cells differentiate into intestinal type cells
Runx3-/- gastric epithelial cells differentiate into intestinal type cells
复制标题
DOI：
10.1016/j.bbrc.2004.06.099
复制
发表时间：
2004-08-11
影响因子：
3.1
通讯作者：
Ito, Y
中科院分区：
文献类型：
--
作者：
Fukamachi, H;Ito, K;Ito, Y
我们此前报道Runx3是一种runt结构域转录因子，是胃上皮细胞的主要生长调节因子，Runx3功能缺失与人胃癌的发生发展有因果关系，Runx3在人胃肠化生中表达显著降低。我们检测了Runx3（-/-）小鼠胃上皮细胞的分化，发现一些细胞分化为肠型细胞，表达Cdx2，这是一种转录因子，已被证明在转基因小鼠中诱导肠化生。Runx3（+/+）胃上皮细胞培养未见肠型细胞分化。这些结果表明，胃上皮细胞能够向肠型细胞分化，可能与Runx3功能受损时Cdx2在胃上皮细胞中的表达有关。讨论Runx3基因功能丧失、肠化生形成与胃癌的关系。(C) 2004爱思唯尔公司版权所有。
We have previously reported that Runx3, a runt domain transcription factor, is a major growth regulator of gastric epithelial cells, that a lack of RUNX3 function is causally related to the genesis and progression of human gastric cancer, and that expression of RUNX3 is greatly reduced in intestinal metaplasias in human stomachs. Here we examined the differentiation of Runx3(-/-) mouse gastric epithelial cells and found that some cells differentiated into intestinal type cells, which expressed Cdx2, a transcription factor that has been shown to induce intestinal metaplasia in transgenic mice. Differentiation of intestinal type cells was not found in culture of Runx3(+/+) gastric epithelial cells. These results suggest that gastric epithelial cells can differentiate into intestinal type cells, probably due to expression of Cdx2 in them when the function of Runx3 is impaired. The relationship between loss of function of Runx3, formation of intestinal metaplasia, and gastric cancer was discussed. (C) 2004 Elsevier Inc. All rights reserved.