Effects of high-fat diet and regular aerobic exercise on global gene expression in skeletal muscle of C57BL/6 mice
Effects of high-fat diet and regular aerobic exercise on global gene expression in skeletal muscle of C57BL/6 mice
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DOI：
10.1016/j.metabol.2011.06.017
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发表时间：
2012-02-01
影响因子：
9.8
通讯作者：
Lavi, Ehud
中科院分区：
文献类型：
--
作者：
Fu, Li;Liu, Xiaolei;Lavi, Ehud
运动训练可以降低胰岛素抵抗（IR）和增加葡萄糖耐量。然而，骨骼肌在分子和遗传水平上的适应性反应还没有被清楚地理解。在这里，我们使用寡核苷酸芯片分析，剖析高脂饮食（HFD）和定期有氧运动对C57 BL/6小鼠骨骼肌的整体基因表达的影响。用正常食物（n = 20）和HFD（n = 20）喂养C57 BL/6雄性小鼠（n = 40）8周。然后将动物分为4个干预组中的1个：正常食物和HFD喂养的小鼠组（NE和HE）和它们的久坐对照组（NC和HC），所述HFD伴随以75%最大耗氧量进行6周的跑台跑步（60 min/d）。应用寡核苷酸芯片技术从转录水平分析有氧运动和HFD的影响，并对筛选出的基因进行实时聚合酶链反应验证。我们的数据表明，6周的有氧运动改善了血脂水平，并逆转了HFD诱导的葡萄糖耐受不良。与NC组相比，HC小鼠样品中的一组503个基因差异表达。通过比较HE和HC组之间表达谱的变化，这些基因中的40个被鉴定为参与有氧运动改善IR的过程。这些变化包括参与代谢、防御和炎症的基因以及功能未知的基因。有氧运动训练能改善HFD维持小鼠的IR。本研究中确定的改善IR的生化途径可能代表IR治疗的潜在靶点。（C）2012 Elsevier Inc. All rights reserved.
Exercise training may decrease insulin resistance (IR) and increase glucose tolerance. However, the adaptive responses in skeletal muscle at the molecular and genetic level have not been clearly understood. Here we used oligonucleotide microarray analysis to dissect the effects of high-fat diet (HFD) and regular aerobic exercise on global gene expression in the skeletal muscle of C57BL/6 mice. C57BL/6 male mice (n = 40) were fed with normal chow (n = 20) and HFD (n = 20) for 8 weeks. The animals were then divided into 1 of 4 intervention groups: groups of mice fed with normal chow and HFD accompanied with 6-week treadmill running (60 min/d) at 75% maximum oxygen consumption (NE and HE) and their sedentary control groups (NC and HC). Oligonucleotide microarray was applied to analyze the effect of aerobic exercise and HFD at the transcriptional level, and selected genes were confirmed by real-time polymerase chain reaction. Our data showed that 6 weeks of aerobic exercise improved the plasma lipid profile and reversed the glucose intolerance induced by HFD. A set of 503 genes was differentially expressed in samples of HC mice as compared with those of the NC group. Forty of those genes were identified as involved in the process of aerobic exercise ameliorating IR by comparing the changes in expression profiles between the HE and HC groups. These changes include genes involved in metabolism, defense, and inflammation and genes of unknown function. Aerobic exercise training is able to ameliorate IR of mice maintained with HFD. The biochemical pathways involved in ameliorating IR identified in this study may represent potential targets for the treatment of IR. (C) 2012 Elsevier Inc. All rights reserved.