FISH analysis of Drosophila melanogaster heterochromatin using BACs and P elements
FISH analysis of Drosophila melanogaster heterochromatin using BACs and P elements
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DOI：
10.1007/s00412-003-0241-9
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发表时间：
2003-07-01
期刊：
影响因子：
1.6
通讯作者：
Dimitri, P
中科院分区：
文献类型：
--
作者：
Corradini, N;Rossi, F;Dimitri, P
果蝇2号和3号染色体的异染色质中含有约30个遗传分析确定的必需基因。在过去的十年中，只有少数这些基因的分子特征，并发现相应的蛋白质编码基因参与重要的细胞功能。此外，一些预测的基因已被确定与多线染色体分裂40，41和80相关的基因组序列的注释，但它们的位置上的异染色质的细胞遗传学图谱仍不确定。为了扩大我们目前的知识，位于异染色质的遗传功能，我们进行了荧光原位杂交（FISH）映射到有丝分裂染色体的9个细菌人工染色体（BAC）携带几个预测的基因和13个P元件插入分配到近端区域的2 R和3L。我们发现22个预测基因映射到2 R的h46区域，8个映射到3L的h47区域。这相当于至少有30个预测基因位于这些异染色质区域，而以前的研究只检测到7个重要基因。最后，另外58个基因定位于常染色质-异染色质过渡区或2 R和3L的近端常染色质。
The heterochromatin of chromosomes 2 and 3 of Drosophila melanogaster contains about 30 essential genes defined by genetic analysis. In the last decade only a few of these genes have been molecularly characterized and found to correspond to protein-coding genes involved in important cellular functions. Moreover, several predicted genes have been identified by annotation of genomic sequence that are associated with polytene chromosome divisions 40, 41 and 80 but their locations on the cytogenetic map of the heterochromatin are still uncertain. To expand our current knowledge of the genetic functions located in heterochromatin, we have performed fluorescence in situ hybridization (FISH) mapping to mitotic chromosomes of nine bacterial artificial chromosomes (BACs) carrying several predicted genes and of 13 P element insertions assigned to the proximal regions of 2R and 3L. We found that 22 predicted genes map to the h46 region of 2R and eight map to the h47 regions of 3L. This amounts to at least 30 predicted genes located in these heterochromatic regions, whereas previous studies detected only seven vital genes. Finally, another 58 genes localize either in the euchromatin-heterochromatin transition regions or in the proximal euchromatin of 2R and 3L.