Fluorescent GD2 analog for single-molecule imaging
Fluorescent GD2 analog for single-molecule imaging
复制标题
用于单分子成像的荧光 GD2 类似物
DOI：
10.1007/s10719-023-10102-1
复制
发表时间：
2023
影响因子：
3
通讯作者：
Ando Hiromune
中科院分区：
文献类型：
--
作者：
Yamaguchi Eriko;Komura Naoko;Tanaka Hide-Nori;Imamura Akihiro;Ishida Hideharu;Groux-Degroote Sophie;M?hlenhoff Martina;Suzuki Kenichi G. N.;Ando Hiromune
神经节苷脂GD2与乳腺癌细胞的增殖和迁移有关。然而，GD2的确切作用尚不清楚，因为它倾向于在细胞质膜（PM）中形成动态和瞬时结构域，称为脂筏，使其难以观察。以前，我们开发了神经节苷脂的荧光类似物（例如，GM3和GM1），这使得首次使用单分子成像观察脂筏形成成为可能。在这份报告中，我们描述了第一个化学合成的荧光神经节苷脂，GD2。合成的类似物的生物物理分析揭示了其筏亲的字符，这表明其潜在的援助单分子成像为基础的调查筏相关的相互作用。
Ganglioside GD2 is associated with the proliferation and migration of breast cancer cells. However, the precise role of GD2 is unclear because its tendency to form dynamic and transient domains in cell plasma membranes (PMs), called lipid rafts, makes it difficult to observe. Previously, we developed fluorescent analogs of gangliosides (e.g., GM3 and GM1), which enabled the observation of lipid raft formation for the first time using single-molecule imaging. In this report, we describe the first chemical synthesis of a fluorescent ganglioside, GD2. A biophysical analysis of the synthesized analog revealed its raft-philic character, suggesting its potential to aid single-molecule imaging-based investigations into raft-associated interactions.