INVITRO ENDOTHELIAL WOUND REPAIR - INTERACTION OF CELL-MIGRATION AND PROLIFERATION
INVITRO ENDOTHELIAL WOUND REPAIR - INTERACTION OF CELL-MIGRATION AND PROLIFERATION
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DOI：
10.1161/01.atv.10.2.215
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发表时间：
1990-03-01
期刊：
影响因子：
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通讯作者：
GOTLIEB, AI
中科院分区：
文献类型：
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作者：
COOMBER, BL;GOTLIEB, AI
大多数再内皮化需要细胞迁移和细胞增殖。为了研究体外伤口模型中细胞迁移和DNA合成起始之间的关联，刮擦在玻璃盖玻片上生长的猪主动脉内皮细胞的汇合培养物以去除单层的一半。用1 ng/ml的紫杉醇（一种微管稳定药物）处理24小时，通过荧光和免疫荧光显微镜评估，F-肌动蛋白或微管组织没有可见的变化。然而，伤口再内皮化减少，中心体向伤口边缘重新分布的细胞比例也相应减少。然而，3 H-胸苷放射自显影显示伤口边缘前两行细胞的标记指数无显著差异。标记的细胞核在103至8和在一个区域内更深的单层减少处理的文化。这些数据表明，与伤口接壤的区域内的细胞中的内皮增殖依赖于剥脱和细胞迁移，剥脱足以促进伤口附近两行中的最大增殖，细胞迁移是从伤口边缘进一步测定细胞增殖传播所需的。
Most re-endothelialization requires both cell migration and cell proliferation. To study the association between cell migration and the initiation of DNA synthesis in an in vitro wound model, confluent cultures of porcine aortic endothelial cells grown on glass coverslips were scraped to remove half of the monolayer. Treatment with 1 ng/ml of taxol, a microtubule stabilizing drug, for 24 hours resulted in no visible change in F-actin or microtubule organization as assessed by fluorescence and immunofluorescence microscopy. There was, however, a reduction of wound re-endothelialization and an associated reduction in the proportion of cells with centrosomes redistributed toward the wound edge. No significant differences, however, were seen in the labeling indices for the first two rows of cells at the wound edge as revealed by 3H-thymidine autoradiography. Labeling of nuclei in Rows 3 to 8 and in a zone deeper within the monolayer was reduced in treated cultures. The data suggest that endothelial proliferation in cells within an area bordering a wound is dependent on both denudation, which is sufficient to promote maximal proliferation in the two rows adjacent to the wound, and cell migration, which is required for the propagation of proliferation in cells further assay from the wound edge.