Genetic mutations associated with chemical resistance in the cytochrome P450 genes of invasive and native Bemisia tabaci (Hemiptera: Aleyrodidae) populations in China
Genetic mutations associated with chemical resistance in the cytochrome P450 genes of invasive and native Bemisia tabaci (Hemiptera: Aleyrodidae) populations in China
复制标题
中国入侵和本地烟粉虱（半翅目：粉虱科）种群细胞色素 P450 基因中与化学抗性相关的基因突变
DOI：
10.1111/j.1744-7917.2009.01253.x
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发表时间：
2009-06-01
期刊：
影响因子：
4
通讯作者：
Ren, Shun-Xiang
中科院分区：
文献类型：
--
作者：
Qiu, Bao-Li;Liu, Li;Ren, Shun-Xiang
烟粉虱 (Gennadius) 是一个复合物种，其两种最具破坏性的生物型 B 和 Q 是全球分布的害虫。尽管生物和经济影响不断增加，但人们对有利于它们与本地种群竞争的进化机制知之甚少。在这里，我们研究了侵入性 B、Q 生物型和本地 Cv 群体的 P450 基因的基因突变。在 B 和 Q 生物型的细胞色素 P450 CYP6C 和 CYP9F 基因中发现了四种与化学抗性相关的突变：Pro-Leu、Ala-Ser、Ser-Phe 和 Trp-Leu。生物测定结果还显示，B和Q生物型对乙酰甲胺磷、高效氯氰菊酯、灭多威的抗性比Cv群体高约12-47倍，对吡虫啉杀虫剂的抗性高约5-7倍。我们的研究结果为更好地了解和监测中国入侵和本地烟粉虱种群的农药抗性提供了一种分子方法。
Bemisia tabaci (Gennadius) is a species complex, and its two most damaging biotypes B and Q are globally distributed pests. Despite increasing biological and economic impacts, little is known about the evolutionary mechanisms that favor their competition with native populations. Here, we investigated the genetic mutations in the P450 gene of the invasive B, Q biotypes and the native Cv population. Four mutations associated with chemical resistance, Pro-Leu, Ala-Ser, Ser-Phe and Trp-Leu, were found in the cytochrome P450 CYP6C and CYP9F genes of the B and Q biotypes. Bioassay results also revealed that both the B and Q biotypes have about 12-47 times more resistance to acephate, beta-cypermethrin, methomyl, and 5-7 times more resistance to imidacloprid insecticide than Cv population. Our results provide a molecular approach for better understanding and monitoring the pesticide resistances of invasive and native B. tabaci populations in China.