Released exopolysaccharide (r-EPS) produced from probiotic bacteria reduce biofilm formation of enterohemorrhagic Escherichia coli O157:H7
Released exopolysaccharide (r-EPS) produced from probiotic bacteria reduce biofilm formation of enterohemorrhagic Escherichia coli O157:H7
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DOI：
10.1016/j.bbrc.2008.12.053
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发表时间：
2009-02-06
影响因子：
3.1
通讯作者：
Kim, Sae Hun
中科院分区：
文献类型：
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作者：
Kim, Younghoon;Oh, Sejong;Kim, Sae Hun
在这里，我们的特点是从嗜酸乳杆菌A4释放的胞外多糖（r-EPS）的目标是确定天然化合物，抑制生物膜的形成。在含有1.0 mg/ml r-EPS的塑料96孔微孔板中，聚苯乙烯和聚氯乙烯（PVC）表面上的肠出血性大肠杆菌（EHEC）生物膜分别显著降低了87%和94%。在r-EPS的存在下，无论是他们的生长速度，也不是他们的autoinducer-2样活性的影响，对肠出血性大肠杆菌O 157：H7。重要的是，当将r-EPS应用于连续流动室模型时，也观察到生物膜形成的一致减少。此外，我们发现添加r-EPS通过影响与curli产生相关的基因（crl，csgA.和csgB）和趋化性（cheY）。此外，这些r-EPS可以防止生物膜形成的革兰氏阴性和阳性病原体。这一特性可能导致开发用于微生物生物膜控制的新型食品级杀虫剂。(c)2008年爱思唯尔公司All rights reserved.
Here, we characterized released-exopolysaccharides (r-EPS) from Lactobacillus acidophilus A4 with the goal of identifying natural compounds that represses biofilm formation. In plastic 96-well microplates that contained 1.0 mg/ml of r-EPS, enterohemorrhagic Escherichia coli (EHEC) biofilms were dramatically decreased by 87% and 94% on polystyrene and polyvinyl chloride (PVC) surfaces, respectively. In the presence of r-EPS, neither their growth rate nor their autoinducer-2-like activity was affected on the EHEC O157:H7. Importantly, consistent reduction in biofilm formation was also observed when r-EPS was applied to the continuous-flow chamber models. In addition, we found that adding r-EPS significantly repressed biofilm formation by affecting genes related to curli production (crl, csgA. and csgB) and chemotaxis (cheY) in transcriptome analysis. Furthermore, these r-EPS Could prevent biofilm formation by a wide range of Gram-negative and -positive pathogens. This property may lead to the development of novel food-grade adjuncts for microbial biofilm control. (c) 2008 Elsevier Inc. All rights reserved.