CHARACTERIZATION OF BACILLUS-SUBTILIS RECOMBINATIONAL PATHWAYS
CHARACTERIZATION OF BACILLUS-SUBTILIS RECOMBINATIONAL PATHWAYS
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DOI：
10.1128/jb.173.13.3977-3980.1991
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发表时间：
1991-07-01
影响因子：
3.2
通讯作者：
TAILOR, RH
中科院分区：
文献类型：
--
作者：
ALONSO, JC;LUDER, G;TAILOR, RH
枯草芽孢杆菌中的重组需要大量重组基因座的产物。 为了剖析可能涉及基因重组的各种机制，我们构建了一系列含有多个突变rec等位基因的等基因菌株。 根据其遗传交换受损情况，各个基因座（由特定的rec等位基因代表）被分为不同的上位组。 α组由recB、recD、recF、recG、recL和recR突变代表的rec基因组成，而β组包括addA和addB突变。 Group-gamma 由recH 和recP 突变组成。 这些结果表明枯草芽孢杆菌具有多种基因重组途径，并且α、β和γ上位组内的基因产物参与这些替代重组途径。 RecA 蛋白是所有三种分子间重组途径所必需的。
Recombination in Bacillus subtilis requires the products of numerous rec loci. To dissect the various mechanisms which may be involved in genetic recombination, we constructed a series of isogenic strains containing more than one mutant rec allele. On the basis of their impairment in genetic exchange, the various loci (represented by specific rec alleles) were classified into different epistatic groups. Group-alpha consists of rec genes represented by recB, recD, recF, recG, recL, and recR mutations, while group-beta comprises the addA and addB mutations. Group-gamma consists of the recH and recP mutations. These results suggest that B. subtilis has multiple pathways for genetic recombination and that the products of the genes within the alpha, beta, and gamma-epistatic groups are involved in these alternative recombination pathways. The RecA protein is required in all three pathways of intermolecular recombination.