Engineering enzymes for biocatalysis.
Engineering enzymes for biocatalysis.
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DOI：
10.2174/187220807779813929
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发表时间：
2007-01-01
影响因子：
--
通讯作者：
Dalby, Paul A
中科院分区：
文献类型：
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作者：
Dalby, Paul A
从基因工程方法的发展开始，蛋白质工程技术已经有二十多年的历史了。从那时起，酶的工程随着在实验室中设计和进化蛋白质的新技术和策略的范围和效率的革命而迅速发展。虽然高通量筛选技术的最新进展允许更快速地筛选更大的酶库，但新的遗传工具和计算方法的结合使针对酶结构区域的随机诱变能够有效地应用，这些区域更有可能引发其生物催化性能的期望增强。与此同时，酶性质的合理设计，特别是计算设计正显示出巨大的潜力。本文综述了近年来与生物催化酶工程相关的重要专利。
Protein engineering techniques have been available for over two decades beginning with the development of methods for genetic engineering. Since that time, the engineering of enzymes has advanced rapidly along with a revolution in the range and efficiency of new techniques and strategies for designing and evolving proteins in the laboratory. While recent advances in high-throughput screening techniques are permitting larger libraries of enzymes to be screened more rapidly, a combination of new genetic tools and computational methods are enabling the efficient application of random mutagenesis targeted to areas of enzyme structures that are more likely to elicit the desired enhancement of their biocatalytic properties. Meanwhile the rational design of enzyme properties, in particular, by computational design is showing great potential. This review article summarises recent and important patents relating to the engineering of enzymes for biocatalysis.