Characterization by electrophoresis of epidermal growth factor stimulated phosphorylation using A-431 membranes.
Characterization by electrophoresis of epidermal growth factor stimulated phosphorylation using A-431 membranes.
复制标题
使用 A-431 膜对表皮生长因子刺激的磷酸化进行电泳表征。
DOI：
10.1021/bi00548a040
复制
发表时间：
1980
期刊：
影响因子：
2.9
通讯作者：
S. Cohen
中科院分区：
文献类型：
--
作者：
L. King;G. Carpenter;S. Cohen
材料与方法材料。小鼠EGF是通过前面描述的程序分离出来的(Savage&Cohen，1972)。CAMP、二丁酰-cAMP、cGMP、三磷酸核苷酸和组蛋白f，来自退伍军人管理局医学中心和田纳西州纳什维尔范德比尔特大学医学院医学(皮肤科)和生物化学系，37203。1979年9月7日收到。这项工作得到了退伍军人管理局、生物医学研究支持补助金RR-50424-16、国家基金会-DIMES三月的Basil O‘Connor研究起步补助金、国家癌症研究所补助金CA-24071以及美国公共卫生服务补助金HD-00700和AM-26518的资金支持。
Materials and Methods Materials. Mouse EGF was isolated by procedures pre-viously described (Savage & Cohen, 1972). cAMP, di-butyryl-cAMP, cGMP, nucleotide triphosphates, and histones f From the Research Service, Veterans Administration Medical Cen-ter, and Departments of Medicine (Dermatology) and Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37203. Received September 7, 1979. This work was supported by funds from the Veterans Administration, Biomedical Research Support Grant RR-50424-16, Basil O’Connor Research Starter Grant from the National Foundation-March of Dimes, National Cancer Institute Grant CA-24071, and US PublicHealth Service Grants HD-00700 and AM-26518.