A mitochondria-targeting near-infrared fluorescent probe for imaging hypochlorous acid in cells
A mitochondria-targeting near-infrared fluorescent probe for imaging hypochlorous acid in cells
复制标题
用于细胞中次氯酸成像的线粒体靶向近红外荧光探针
DOI：
10.1016/j.talanta.2021.122152
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发表时间：
2021
期刊：
影响因子：
6.1
通讯作者：
Tang Bo
中科院分区：
文献类型：
--
作者：
Zheng Aishan;Liu Han;Peng Chuanhao;Gao Xiaonan;Xu Kehua;Tang Bo
Hypochlorous acid (HOCl), as an important reactive oxygen species (ROS), plays an important role in various pathological and physiological processes. Therefore, it is of great significance to identify and detect HOCl in organisms. Herein, a mitochondria-targeting near-Infrared fluorescent probe (CVS) has been designed and synthesized for sensing HOCl. Under simulated physiological conditions, CVS can be instantly oxidized by HOCl to CVSdouble bondO and the fluorescence signal is significantly enhanced. Additionally, CVS shows high selectivity toward HOCl over other ROS and owns low detection limit (94.7 nM) for HOCl. Moreover, CVS can be successfully applied to image HOCl in mitochondria of the HepG2 cells. Thus, CVS is a powerful tool for sensing HOCl and provides a perspective method for further study of the physiological and pathological functions related to HOCl.
