Measurement of bone blood now using the hydrogen washout technique - Part II: Validation by comparison to microsphere entrapment
Measurement of bone blood now using the hydrogen washout technique - Part II: Validation by comparison to microsphere entrapment
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DOI：
10.1002/jor.20561
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发表时间：
2008-06-01
影响因子：
2.8
通讯作者：
Bishop, Allen T.
中科院分区：
文献类型：
--
作者：
Larsen, Mikko;Pelzer, Michael;Bishop, Allen T.
骨血流量的精确和可重复性测量具有重要的临床和实验应用。氢冲洗法简单、安全、应用广泛，但其在骨组织中的应用尚未得到验证。为此，我们比较了放射性标记微球包埋法和氢冲洗法获得的皮质骨血流测量结果。采用放射性微球包埋法和氢洗法测定12只新西兰大白兔胫骨皮质骨的血流量。除对照组（n = 6）外，4只动物给予全身肾上腺素（0.8 μ g/kg/min）治疗（2组），2只动物给予硝普苷（100 μ g/kg/min）治疗（3组）。此外，取7只大鼠的9根股骨，在其血管蒂上分离，用氢冲洗法在每个位置重复测量骨血流量，以确认氢冲洗法测定血流的可重复性。微球法的平均流速为2.3 +/- 2.0 mL/min/ 100g，氢冲洗法的平均流速为2.0 +/- 0.5 mL/min/ 100g。相关性和一致性显著：R-2 = 0.97 （p < 0.01）。在给药过程中没有发现血流变化的一致性。氢冲洗提供了可重复的结果，并显示出对流量变化的高灵敏度。氢冲洗法测量骨血流量具有敏感性和可重复性。结果与标记微球包埋得到的流量值吻合良好。2008年骨科研究学会年会。Wiley期刊公司出版。
Accurate and reproducible measurement of bone blood flow has important clinical and experimental applications. Hydrogen washout is simple, safe, and widely used, but its use in bone tissue has not been validated. To this end, we have compared cortical bone blood flow measurements obtained by radioactive-labeled microsphere entrapment with those from hydrogen washout. Blood flow was measured in tibial cortical bone of 12 New Zealand White rabbits by radioactive microsphere entrapment and by hydrogen washout. Besides a control group (n = 6), four animals were treated with systemic epinephrine (0.8 mu g/kg/min) (group 2) and two with nitroprusside (100 mu g/kg/min) (group 3). Furthermore, nine femora from seven rats were isolated on their vascular pedicles and repeated bone blood flow measurements were made at each location with the hydrogen washout method to confirm reproducibility of blood flow determinations by hydrogen washout. An average flow of 2.3 +/- 2.0 mL/min/100 g was obtained with the microsphere method and 2.0 +/- 0.5 mL/min/100 g with the hydrogen washout method. There was a significant correlation and agreement: R-2 = 0.97 (p < 0.01). No consistent flow variations were found with systemic vasoactive drug administration. Hydrogen washout provided reproducible results and showed high sensitivity to flow changes. Hydrogen washout is both sensitive and reproducible in measuring bone blood flow. Results agree well with flow values obtained by labeled microsphere entrapment. 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.