Systematic analyses reveal uniqueness and origin of the CFEM domain in fungi.
Systematic analyses reveal uniqueness and origin of the CFEM domain in fungi.
复制标题
系统分析揭示真菌中 CFEM 结构域的独特性和起源
DOI：
10.1038/srep13032
复制
发表时间：
2015-08-10
影响因子：
4.6
通讯作者：
Zou CG
中科院分区：
文献类型：
--
作者：
Zhang ZN;Wu QY;Zhang GZ;Zhu YY;Murphy RW;Liu Z;Zou CG
CFEM结构域通常发生在真菌外膜蛋白中。为了提供理解CFEM推定功能的见解，我们通过在各种动物，植物和100多种真菌物种之间进行系统的比较基因组分析来研究CFEM域的进化动力学，这些分析是整个真菌组的代表性。我们在这里表明CFEM域是真菌独有的。使用组织培养的实验表明，某些植物中含有CFEM的EST源自内生真菌。我们还发现CFEM域并非发生在所有真菌中。它的单一起源可追溯到commycota和basidiomycota的最新共同祖先，而不是多个起源。尽管CFEM结构域的长度和结构相对保守，但在不同的真菌物种之间，域名数差异很大。通常，与其他物种相比，致病真菌具有更多的域。跨真菌基因组的域扩展似乎是由域的重复和基因复制驱动的。这些发现产生了CFEM结构域的明确进化轨迹，并提供了从细胞表面成分到宿主 - 病原体相互作用中介质的含CFEM蛋白功能交换的新见解。
CFEM domain commonly occurs in fungal extracellular membrane proteins. To provide insights for understanding putative functions of CFEM, we investigate the evolutionary dynamics of CFEM domains by systematic comparative genomic analyses among diverse animals, plants and more than 100 fungal species, which are representative across the entire group of fungi. We here show that CFEM domain is unique to fungi. Experiments using tissue culture demonstrate that the CFEM-containing ESTs in some plants originate from endophytic fungi. We also find that CFEM domain does not occur in all fungi. Its single origin dates to the most recent common ancestors of Ascomycota and Basidiomycota, instead of multiple origins. Although the length and architecture of CFEM domains are relatively conserved, the domain-number varies significantly among different fungal species. In general, pathogenic fungi have a larger number of domains compared to other species. Domain-expansion across fungal genomes appears to be driven by domain duplication and gene duplication via recombination. These findings generate a clear evolutionary trajectory of CFEM domains and provide novel insights into the functional exchange of CFEM-containing proteins from cell-surface components to mediators in host-pathogen interactions.