Improvement of Endurance Based on Muscle Fiber-Type Composition by Treatment with Dietary Apple Polyphenols in Rats.
Improvement of Endurance Based on Muscle Fiber-Type Composition by Treatment with Dietary Apple Polyphenols in Rats.
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DOI：
10.1371/journal.pone.0134303
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Tatsumi R
中科院分区：
文献类型：
--
作者：
Mizunoya W;Miyahara H;Okamoto S;Akahoshi M;Suzuki T;Do MK;Ohtsubo H;Komiya Y;Lan M;Waga T;Iwata A;Nakazato K;Ikeuchi Y;Anderson JE;Tatsumi R
最近的一项研究表明，苹果多酚（APP）摄入量对成年动物的肌肉耐力有积极影响。虽然脂质代谢的增强可能是改善的部分原因，但其作用机制仍需澄清。在这里，我们表明，8周的摄入量5%（w/w）APP的饮食，上调两个功能相关的纤维类型：肌球蛋白重链（MyHC）类型IIx/IIb和肌红蛋白蛋白表达的比例在9周龄的雄性Fischer F344大鼠的跖肌相比，配对喂养的控制（P < 0.05）。结果通过我们专门用于MyHC亚型分离的SDS-PAGE系统和全肌肉的蛋白质印迹法证明。对照组和5% APP喂养动物（n = 9只/组）之间的动物生长特征（摄食量、体重增量和内脏器官重量）无差异。研究结果可以解释下肢肌肉的抗疲劳性的增加，如由胫神经的100-s电刺激序列产生的最大等长跖屈扭矩的缓慢下降所证明的。此外，8周0.5%APP饮食后的抗疲劳性低于5%APP饮食后的抗疲劳性，支持纤维类型组成变化的APP剂量依赖性。因此，本研究强调了一个有前途的贡献，饮食APP摄入量增加耐力的基础上纤维型组成的大鼠肌肉。研究结果可能有助于开发一种新的策略，用于动物科学、人类运动和与年龄相关的健康科学。
A recent study demonstrated a positive effect of apple polyphenol (APP) intake on muscle endurance of young-adult animals. While an enhancement of lipid metabolism may be responsible, in part, for the improvement, the contributing mechanisms still need clarification. Here we show that an 8-week intake of 5% (w/w) APP in the diet, up-regulates two features related to fiber type: the ratio of myosin heavy chain (MyHC) type IIx/IIb and myoglobin protein expression in plantaris muscle of 9-week-old male Fischer F344 rats compared to pair-fed controls (P < 0.05). Results were demonstrated by our SDS-PAGE system specialized for MyHC isoform separation and western blotting of whole muscles. Animal-growth profiles (food intake, body-weight gain, and internal-organ weights) did not differ between the control and 5% APP-fed animals (n = 9/group). Findings may account for the increase in fatigue resistance of lower hind limb muscles, as evidenced by a slower decline in the maximum isometric planter-flexion torque generated by a 100-s train of electrical stimulation of the tibial nerve. Additionally, the fatigue resistance was lower after 8 weeks of a 0.5% APP diet than after 5% APP, supporting an APP-dose dependency of the shift in fiber-type composition. Therefore, the present study highlights a promising contribution of dietary APP intake to increasing endurance based on fiber-type composition in rat muscle. Results may help in developing a novel strategy for application in animal sciences, and human sports and age-related health sciences.