Growth-dependent changes in elemental stoichiometry and macromolecular allocation in the coccolithophore Emiliania huxleyi under different environmental conditions
Growth-dependent changes in elemental stoichiometry and macromolecular allocation in the coccolithophore Emiliania huxleyi under different environmental conditions
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不同环境条件下颗石藻艾米利亚赫胥黎元素化学计量和大分子分配的生长依赖性变化
DOI：
10.1002/lno.11854
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发表时间：
2021
影响因子：
4.5
通讯作者：
Finkel Zoe V.
中科院分区：
文献类型：
--
作者：
Zhang Yong;Li Zhengke;Schulz Kai G.;Hu Yingyu;Irwin Andrew J.;Finkel Zoe V.
生长速率假说（GRH）假定随着生长速率的增加，核糖体核糖核酸（RNA）含量增加，因此细胞磷（P）也增加。有证据表明，GRH可能不适用于所有条件下的浮游植物。在这里，我们实验控制四个条件（光，温度，pH值，和CO2），以改变的增长率ofEmiliania huxleyi，一个地球化学重要的颗石藻，并监测RNA，蛋白质和碳水化合物含量的变化。我们发现，增加光照，pH值，和CO2引起的增长率增加，导致每单位有机碳的RNA（RNA：POC）增加，但增加温度，导致增长率的增加，导致RNA：POC减少。每单位有机碳的蛋白质（蛋白质：POC）增加，在我们增加的温度，pH值，和CO2的处理，增加了生长速率，但在我们的光处理的蛋白质：POC的变化不大，尽管它诱导相同的增长率。每单位有机碳的碳水化合物（碳水化合物：POC）增加的增长率下增加的光和CO2，但没有显着变化的温度或pH值的处理。这些结果表明，生理适应特定的环境条件下，可以导致RNA，蛋白质和碳水化合物组成的对比模式，因此对比变化的碳：氮：磷比例与生长速度在E。huxleyi。
The growth rate hypothesis (GRH) posits an increase in ribosomal ribonucleic acid (RNA) content, and therefore cellular phosphorus (P), with increasing growth rate. There is evidence that the GRH may not apply to phytoplankton under all conditions. Here, we experimentally controlled four conditions (light, temperature, pH, and CO2) to alter the growth rate ofEmiliania huxleyi, a biogeochemically important coccolithophorid, and monitored changes in RNA, protein, and carbohydrate content. We show that an increase in growth rate caused by increasing light, pH, and CO2resulted in increased RNA per unit of organic carbon (RNA : POC), but that increasing temperature, leading to increase of growth rate, resulted in a decrease in RNA : POC. Protein per unit of organic carbon (protein : POC) increased in our increased temperature, pH, and CO2treatments that increased growth rate, but there was little change in protein : POC in our light treatment despite it inducing the same increase in growth rate. Carbohydrate per unit of organic carbon (Carbohydrate : POC) increased with growth rate under increased light and CO2but did not vary significantly in the temperature or pH treatments. These results indicate that physiological acclimation to specific environmental conditions can lead to contrasting patterns in RNA, protein, and carbohydrate composition and therefore contrasting changes in carbon : nitrogen : phosphorus ratios with growth rate inE. huxleyi.