Developmental reorganization of the skeletal framework and its surface lamina in fusing muscle cells.
Developmental reorganization of the skeletal framework and its surface lamina in fusing muscle cells.
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DOI：
10.1083/jcb.91.1.103
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发表时间：
1981-10
期刊：
影响因子：
--
通讯作者：
Penman S
中科院分区：
文献类型：
--
作者：
Fulton AB;Prives J;Farmer SR;Penman S
用洗涤剂提取后，细胞的骨骼框架由内部结构纤维、微小梁和表面层组成，非常清晰。当肌肉细胞融合形成多核肌管时，它们的骨骼框架会广泛重组。当成肌细胞准备融合时，先前连续的表面层会形成许多此阶段特有的空隙。碘化表面蛋白的保留表明腔隙不是通过提取层蛋白而形成的。这些凹陷似乎对应于不结合刀豆球蛋白 A 的广泛斑块，并且可能是缺乏糖蛋白的脂质双层区域。这些陷窝似乎与融合有关，并在多核肌管形成后迅速消失。当肌肉细胞融合时，它们的内部结构网络必须互连以形成肌管的框架。整个骨骼框架的透射电子显微镜显示，增殖的成肌细胞具有发育良好且高度互连的内部网络。在融合之前，这些网络会被广泛重组和不稳定。融合后，形成稳定的、广泛交联的内部结构，但具有肌管的形态特征。因此，肌肉细胞在融合时在表面和内部都会经历广泛的重组。
The skeletal framework of cells, composed of internal structural fibers, microtrabeculae, and the surface lamina, is revealed with great clarity after extraction with detergent. When muscle cells fuse to form a multinucleated myotube, their skeletal framework reorganizes extensively. When myoblasts prepare to fuse, the previously continuous surface lamina develops numerous lacunae unique to this stage. The retention of iodinated surface proteins suggests that the lacunae are not formed by the extraction of lamina proteins. The lacunae appear to correspond to extensive patches that do not bind concanavalin A and are probably regions of lipid bilayer devoid of glycoproteins. The lacunae appear to be related to fusion and disappear rapidly after the multinucleated myotube is formed. When muscle cells fuse, their internal structural networks must interconnect to form the framework of the myotube. Transmission electron microscopy of skeletal framework whole mounts shows that proliferating myoblasts have well developed and highly interconnected internal networks. Immediately before fusion, these networks are extensively reorganized and destabilized. After fusion, a stable, extensively cross-linked internal structure is reformed, but with a morphology characteristic of the myotube. Muscle cells therefore undergo extensive reorganization both on the surface and internally at the time of fusion.