A transgenic mouse strain expressing four drug-selectable marker genes
A transgenic mouse strain expressing four drug-selectable marker genes
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DOI：
10.1093/nar/25.18.3745
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发表时间：
1997-09-15
影响因子：
14.9
通讯作者：
Jaenisch, R
中科院分区：
文献类型：
--
作者：
Tucker, KL;Wang, YK;Jaenisch, R
小鼠胚胎干细胞(ES)通常在原代小鼠胚胎成纤维细胞(MEF)饲养层上培养。由于基因打靶实验经常涉及单个ES细胞系中多药耐药的序贯选择，我们开发了一种新的小鼠品系，它代表了生产多药耐药MEF的经济供体。从DR-4小鼠品系制备的MEF对药物G418、6-硫鸟嘌呤、嘌呤霉素和潮霉素的抗药性远远高于通常用于选择耐药ES细胞的浓度。
Murine embryonic stem (ES) cells are commonly cultured on feeder layers of primary murine embryonic fibroblasts (MEFs). Because gene targeting experiments often involve sequential selection for multiple-drug resistance in single ES cell lines, we have developed a new mouse strain which represents an economical donor for the production of multiple-drug resistant MEFs. MEFs prepared from the DR-4 mouse strain displayed resistance to concentrations of the drugs G418, 6-thioguanine, puromycin and hygromycin well above those used normally for the selection of drug-resistant ES cells.