Immunohistochemistry for the diagnosis of hepatitis E virus infection
Immunohistochemistry for the diagnosis of hepatitis E virus infection
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DOI：
10.1111/j.1365-2893.2011.01498.x
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发表时间：
2012-02-01
影响因子：
2.5
通讯作者：
Panda, S. K.
中科院分区：
文献类型：
--
作者：
Gupta, P.;Jagya, N.;Panda, S. K.
.戊型肝炎病毒（Hepatitis E virus，HEV）是一种新兴的病原体，也是世界范围内急性病毒性肝炎的最常见病因。在这里，我们描述了一种免疫组织化学方法检测戊型肝炎病毒抗原（pORF 2和pORF 3）在福尔马林固定，石蜡包埋的肝组织中使用单克隆抗体提出对两个病毒蛋白（pORF 2和pORF 3）。在急性肝衰竭（ALF）病例中，我们分析了它们的特异性和敏感性，并与血清学和核酸检测进行比较。我们用此试验对30例戊型肝炎病毒感染引起的ALF患者的尸检肝组织进行了检测。这些病例是根据酶免疫测定（IgM抗-HEV）的阳性结果选择的。从病理科档案中提取的30个病例中，抗体成功地染色了所有病例。其中25份（83.3%）血清标本呈阳性。使用的15个对照（5个未感染的肝组织，5个HBV和5个丙型肝炎病毒感染的肝组织）均为阴性。免疫组化检测可能证明是一个有价值的工具，用于检测HEV感染的活检，尸检和移植肝组织，并可以作为一个链接沿着与其他可用的测试，以描绘的程度HEV相关的问题，世界范围内。
. Hepatitis E virus (HEV) is an emerging pathogen and the most common cause of acute viral hepatitis all over the world. We describe here an immunohistochemical method for the detection of HEV antigens (pORF2 and pORF3) in formalin-fixed, paraffin-embedded liver tissues using monoclonal antibodies raised against two of the virus proteins (pORF2 and pORF3). We analysed their specificity and sensitivity in comparison with serology and nucleic acid detection in cases of acute liver failure (ALF). We used this test on 30 liver biopsies collected post-mortem from the patients of ALF caused by HEV infection. These cases were selected on the basis of positive results for enzyme immunoassay (IgM anti-HEV). Of the 30 cases taken from the archives of the Department of Pathology, the antibodies successfully stained all. However, only 25 serum samples (83.3%) of these were positive for HEV RNA. Fifteen controls used (Five noninfected liver tissues, five HBV- and five hepatitis C virus-infected liver tissues) were all negative. The immunohistochemical assay described here may prove a valuable tool for the detection of HEV infection in biopsy, autopsy and explant liver tissues and can serve as a link along with other available tests to delineate the extent of HEV-associated problem worldwide.