Size-Dependent Cell Uptake of Protein-Coated Graphene Oxide Nanosheets
Size-Dependent Cell Uptake of Protein-Coated Graphene Oxide Nanosheets
复制标题
蛋白质涂层氧化石墨烯纳米片的尺寸依赖性细胞摄取
DOI：
10.1021/am300253c
复制
发表时间：
2012-04-01
影响因子：
9.5
通讯作者：
Yan, Bing
中科院分区：
文献类型：
--
作者：
Mu, Qingxin;Su, Gaoxing;Yan, Bing
石墨烯作为一种新兴的应用材料，在包括生物医学在内的诸多领域都显示出巨大的应用潜力。然而，这些纳米片的生物学行为，特别是它们与细胞的相互作用，还没有得到很好的理解。在这里，我们报告我们的研究结果的细胞表面粘附，亚细胞的位置，和蛋白质涂层的氧化石墨烯纳米片（PCGO）的大小依赖的摄取机制。小的纳米片主要通过网格蛋白介导的内吞作用进入细胞，并且石墨烯尺寸的增加增强了纳米片的吞噬摄取。这些发现将促进石墨烯的生物医学和毒理学研究，并提供二维纳米结构和生物系统界面相互作用的基本理解。
As an emerging applied material, graphene has shown tremendous application potential in many fields, including biomedicine. However, the biological behavior of these nanosheets, especially their interactions with cells, is not well understood. Here, we report our findings about the cell surface adhesion, subcellular locations, and size-dependent uptake mechanisms of protein-coated graphene oxide nanosheets (PCGO). Small nanosheets enter cells mainly through clathrin-mediated endocytosis, and the increase of graphene size enhances phagocytotic uptake of the nanosheets. These findings will facilitate biomedical and toxicologic studies of graphenes and provide fundamental understanding of interactions at the interface of two-dimensional nanostructures and biological systems.