A novel monoclonal antibody against mouse B7-H3 developed in rats.
A novel monoclonal antibody against mouse B7-H3 developed in rats.
复制标题
DOI：
10.1089/hyb.2012.0008
复制
发表时间：
2012-08
期刊：
影响因子：
--
通讯作者：
R. Yan;Shun Yang;Jing Sun;Xu-qin Chen;Guangbo Zhang;P. Feng;Xueguang Zhang
中科院分区：
文献类型：
--
作者：
R. Yan;Shun Yang;Jing Sun;Xu-qin Chen;Guangbo Zhang;P. Feng;Xueguang Zhang
B7-H3是B7超家族的新成员，在T细胞活化过程中起着关键作用;其功能尚不清楚。本研究获得了一株新的抗小鼠B7-H3单克隆抗体，并对其生物学功能进行了初步研究。我们的结果表明，该单克隆抗体可用于流式细胞术和免疫印迹和免疫组织化学分析，表明该单克隆抗体的性能比商业单克隆抗体（M3.2D7）好得多。此外，数据显示小鼠B7-H3在各种免疫细胞上的不同表达谱。我们进一步表明，小鼠B7-H3蛋白不表达在正常组织中，除了膀胱上皮细胞使用该单克隆抗体。有趣的是，单克隆抗体可以刺激T细胞的增殖和细胞因子的分泌。该单克隆抗体对进一步研究B7-H3分子具有重要价值。
B7-H3, a novel member of the B7 superfamily, plays a critical role during T cell activation; its functions are still unclear. In this study we obtained a novel anti-mouse B7-H3 monoclonal antibody (MAb) and characterized its biological functions. Our results demonstrated that this MAb could be used for flow cytometry and Western blot and immunohistochemistry analyses, suggesting that the performance of this MAb is much better than a commercial MAb (M3.2D7). Furthermore, data showed different expression profiles of mouse B7-H3 on various immune cells. We further showed that mouse B7-H3 protein was not expressed on normal tissues except for bladder epithelial cells using this MAb. Interestingly, the MAb could stimulate the proliferation and cytokine secretion of T cells. Taken together, this MAb might be of great value for further investigation of B7-H3 molecule.