Six-Helix Bundle and Triangle DNA Origami Insulator-Based Dielectrophoresis
Six-Helix Bundle and Triangle DNA Origami Insulator-Based Dielectrophoresis
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DOI：
10.1021/ac402493u
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发表时间：
2013-12-03
影响因子：
7.4
通讯作者：
Ros, Alexandra
中科院分区：
文献类型：
--
作者：
Gan, Lin;Chao, Tzu-Chiao;Ros, Alexandra
自组装DNA纳米结构在纳米电子电路、靶向药物输送和智能传感方面具有巨大的潜力。它们的应用需要合适的操作方法和纳米级的组装，以及足够的浓缩、纯化和分离方法。基于绝缘体的介电泳法(IDEP)为微米和纳米级物体的操作提供了一种高效且无基质的方法。为了开发用于DNA纳米组装的IDEP，对潜在的极化和介电迁移的详细了解是必不可少的。在这里，我们探索了序列相同但拓扑差异较大的六螺旋DNA原基和三角形DNA原基的介电行为，揭示了IDEP捕获的特征频率范围。此外，将三角形折纸限制在IDEP陷阱中需要更大的外加电场。为了解释观察到的DEP迁移和捕获，我们根据这两个物种的结构差异讨论了它们的极化率模型，并进行了数值模拟，揭示了DNA折纸物种在IDEP捕获区域的电泳运输的贡献。在较低频率下，数值模型与实验结果吻合较好。然而，实验观察到的IDEP陷阱区域的扩展在较高的频率下有相当大的偏差。我们的研究首次证明了DNA折纸物种可以成功地被IDEP捕获和操纵，并揭示了这两种DNA折纸物种独特的IDEP行为。实验中观察到的捕获机制将有助于未来探索DNA折纸操作和组装在纳米和微米尺度上，以及这些纳米组件与IDEP的其他应用。
Self-assembled DNA nanostructures have large potential for nanoelectronic circuitry, targeted drug delivery, and intelligent sensing. Their applications require suitable methods for manipulation and nanoscale assembly as well as adequate concentration, purification, and separation methods. Insulator-based dielectrophoresis (iDEP) provides an efficient and matrix-free approach for manipulation of micro- and nanometer-sized objects. In order to exploit iDEP for DNA nanoassemblies, a detailed understanding of the underlying polarization and dielectrophoretic migration is essential. Here, we explore the dielectrophoretic behavior of six-helix bundle and triangle DNA origamis with identical sequence but large topological difference and reveal a characteristic frequency range of iDEP trapping. Moreover, the confinement of triangle origami in the iDEP trap required larger applied electric fields. To elucidate the observed DEP migration and trapping, we discuss polarizability models for the two species according to their structural difference complemented by numerical simulations, revealing a contribution of the electrophoretic transport of the DNA origami species in the iDEP trapping regions. The numerical model showed reasonable agreement with experiments at lower frequency. However, the extension of the iDEP trapping regions observed experimentally deviated considerably at higher frequencies. Our study demonstrates for the first time that DNA origami species can be successfully trapped and manipulated by iDEP and reveals distinctive iDEP behavior of the two DNA origamis. The experimentally observed trapping regimes will facilitate future exploration of DNA origami manipulation and assembly at the nano- and microscale as well as other applications of these nanoassemblies with iDEP.