EFFECTS OF CLOSTRIDIUM-PERFRINGENS RECOMBINANT AND CRUDE PHOSPHOLIPASE-C AND THETA-TOXIN ON RABBIT HEMODYNAMIC PARAMETERS
EFFECTS OF CLOSTRIDIUM-PERFRINGENS RECOMBINANT AND CRUDE PHOSPHOLIPASE-C AND THETA-TOXIN ON RABBIT HEMODYNAMIC PARAMETERS
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DOI：
10.1093/infdis/172.5.1317
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发表时间：
1995-11-01
影响因子：
6.4
通讯作者：
STEVENS, DL
中科院分区：
文献类型：
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作者：
ASMUTH, DM;OLSON, RD;STEVENS, DL
产气荚膜梭菌外毒素已被认为是导致气性坏疽休克和器官衰竭的主要毒力因子，但它们介导心血管功能障碍的机制(S)仍是个谜。给清醒兔静脉注射重组磷脂酶C(PLC)、重组毒素、培养上清(粗毒素)或0.9%氯化钠，分别于注射毒素前1h和注射后3h测定心脏指数(CI)、平均动脉压(MAP)、心率(HR)、中心静脉压(CVP)、动脉血气和红细胞压积。粗毒素和RPLC可降低CI、MAP和HR，增加CVP，死亡率分别为87.5%和83%。F-theta毒素不降低CI或MAP，死亡率为25%。此外，粗毒素和RPLC可抑制离体兔心房肌的收缩功能(df/dt)，提示PLC引起的心肌功能障碍是产气荚膜梭菌感染休克的原因之一。
Clostridium perfringens exotoxins have been implicated as major virulence factors responsible for shock and organ failure in gas gangrene, yet the mechanism(s) by which they mediate cardiovascular dysfunction remain enigmatic. Recombinant (r) phospholipase C (PLC), re-toxin, culture supernatant (crude toxin), or 0.9% NaCl was infused intravenously into awake rabbits, Cardiac index (CI), mean arterial pressure (MAP), heart rate (HR), central venous pressure (CVP), arterial blood gases, and hematocrit were measured 1 h before and for 3 h after toxin infusion. Crude toxin and rPLC decreased CI, MAP, and HR and increased CVP; mortality was 87.5% and 83%, respectively. f theta-toxin did not decrease CI or MAP and mortality was 25%. Further, crude toxin and rPLC but not re-toxin inhibited cardiac contractility (dF/dt) in isolated rabbit atrial muscles, These results suggest that PLC-induced myocardial dysfunction contributes to shock in C. perfringens infection.