MAP-30 - A NEW INHIBITOR OF HIV-1 INFECTION AND REPLICATION
MAP-30 - A NEW INHIBITOR OF HIV-1 INFECTION AND REPLICATION
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DOI：
10.1016/0014-5793(90)80438-o
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发表时间：
1990-10-15
期刊：
影响因子：
3.5
通讯作者：
HUANG, PL
中科院分区：
文献类型：
--
作者：
LEEHUANG, S;HUANG, PL;HUANG, PL
从苦瓜种子和果实中分离纯化了一种新的HIV抑制剂。这种化合物MAP 30（Momorea Anti-HIV Protein）是一种约30 kDa的碱性蛋白质。其对无细胞HIV-1感染和复制表现出剂量依赖性抑制作用，通过以下指标测定：（i）CEM-ss单层上定量局灶性合胞体形成;（II）病毒核心蛋白p24表达;和（iii）HIV-1感染H9细胞中病毒相关逆转录酶（RT）活性。在这些试验中，50%抑制（ID 50）所需的剂量分别为0.83、0.22和0.33 nM。在试验条件下未发现细胞毒性或细胞抑制效应。这些数据表明MAP 30可能是治疗HIV-1感染的有用治疗剂。已测定MAP 30的N-末端44个氨基酸的序列。
A new inhibitor of human immunodeficiency virus (HIV) has been isolated and purified to homogeneity from the seeds and fruits of theMomordica charantia. This compound, MAP 30 (Momordica Anti-HIV Protein), is a basic protein of about 30 kDa. It exhibits dose-dependent inhibition of cell-free HIV-1 infection and replication as measured by: (i) quantitative focal syncytium formation on CEM-ss monolayers; (II) viral core protein p24 expression; and (iii) viral-associated reverse transcriptase (RT) activity in HIV-1 infected H9 cells. The doses required for 50% inhibition (ID50) in these assays were 0.83, 0.22 and 0.33 nM, respectively. No cytotoxic or cytostatic effects were found under the assay conditions. These data suggest that MAP 30 may be a useful therapeutic agent in the treatment of HIV-1 infections. The sequence of the N-terminal 44 amino acids of MAP 30 has been determined.