Altered Expression of NDRG2 in the Testes of Experimental Rat Model of Cryptorchidism
Altered Expression of NDRG2 in the Testes of Experimental Rat Model of Cryptorchidism
复制标题
DOI：
10.1016/j.urology.2009.05.032
复制
发表时间：
2010-04-01
期刊：
影响因子：
2.1
通讯作者：
Zhang, Yuanqiang
中科院分区：
文献类型：
--
作者：
Hou, Wugang;Hu, Jing;Zhang, Yuanqiang
目的 探讨隐睾大鼠模型中N-Myc下游调节基因2（NDRG2）的表达及其与生殖细胞凋亡的潜在关系。方法对青春期Sprague-Dawley大鼠进行手术诱导实验性单侧隐睾。测量腹部和阴囊睾丸的重量，并通过末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记、免疫组织化学和免疫荧光分析纵向检查其凋亡细胞和NDRG2表达。采用Western blot法检测纯化的生殖细胞和Leydig细胞中NDRG2和p53的表达。结果与对照阴囊睾丸相比，术后7天和14天腹部睾丸的平均重量显着降低，同时凋亡的生殖细胞数量显着增加。尽管在阴囊睾丸的间质细胞和自发凋亡的生殖细胞中检测到了 NDRG2 的表达，但它主要在腹部睾丸的凋亡精母细胞和圆形精子细胞中显着上调。然而，腹部睾丸 Leydig 细胞中 NDRG2 表达下调。 Western blot分析显示，术后7 d纯化的生殖细胞中NDRG2和p53的表达显着上调，而术后7和14 d腹睾纯化的Leydig细胞中NDRG2的表达下调。结论 NDRG2表达上调与隐睾睾丸生殖细胞凋亡相关，提示NDRG2可能是大鼠隐睾生殖细胞凋亡的正调节因子。泌尿学 75: 985-991, 2010。(C) 2010 Elsevier Inc.
OBJECTIVES To examine the expression of N-Myc downstream regulated gene 2 (NDRG2) in the cryptorchid testes and its potential association with germ cell apoptosis in a rat model of cryptorchidism.METHODS Groups of pubertal Sprague-Dawley rats were subjected to surgical procedure for the induction of experimental unilateral cryptorchidism. The weight of the abdominal and scrotal testes was measured and their apoptotic cells and NDRG2 expression were examined longitudinally by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling, immunohistochemistry, and immunofluorescence analyses. The expression of NDRG2 and p53 in the purified germ cells and Leydig cells was determined by Western blot assay.RESULTS Compared with the control scrotal testes, the mean weights of the abdominal testes were significantly reduced at 7 and 14 days after surgery, accompanied by significantly increased numbers of apoptotic germ cells. Although the NDRG2 expression was detected in Leydig cells and spontaneous apoptotic germ cells in the scrotal testes, it was significantly upregulated predominately in apoptotic spermatocytes and round spermatids in the abdominal testes. However, the NDRG2 expression was downregulated in Leydig cells in the abdominal testes. Western blot analysis revealed that the expression of NDRG2 and p53 was upregulated significantly in purified germ cells 7 days after surgery, whereas NDRG2 was downregulated in the purified Leydig cells from the abdominal testes 7 and 14 days after surgery.CONCLUSIONS The upregulated expression of NDRG2 was associated with germ cell apoptosis in the cryptorchid testes, suggesting that the NDRG2 may be a positive regulator for germ cell apoptosis in rat cryptorchidism. UROLOGY 75: 985-991, 2010. (C) 2010 Elsevier Inc.