Process research of macroporous resin chromotography for separation of N-(p-coumaroyl)serotonin and N-feruloylserotonin from Chinese safflower seed extracts
Process research of macroporous resin chromotography for separation of N-(p-coumaroyl)serotonin and N-feruloylserotonin from Chinese safflower seed extracts
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DOI：
10.1016/j.seppur.2008.02.007
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发表时间：
2008-09-01
影响因子：
8.6
通讯作者：
Qiu, Aiyong
中科院分区：
文献类型：
--
作者：
Jin, Qingzhe;Yue, Jinhuan;Qiu, Aiyong
N-（p-香豆酰）5-羟色胺（CS）和N-阿魏酰5-羟色胺（FS）是红花（Carthamus tinctorius L.）种子具有多种生物学效应。本研究对D312、860021、DM 131、HZ 801、AB 8和XDA-1等6种常用大孔吸附树脂的吸附和解吸性能进行了评价和比较，并对CS和FS的富集和制备分离进行了研究。静态吸附和脱附实验表明，XDA-1对CS和FS的吸附和脱附平衡最好，其吸附平衡符合Langmuir等温式。采用XDA-1树脂填充柱进行动态吸附和解吸实验，确定最佳工艺条件为：上样液中CS和FS浓度分别为0.346和0.276 mg/mL，上样量为5倍柱体积，上样流速为2 mL/min，温度为25洗脱液80%乙醇，11 BV，流速1 mL/min（用于解吸）。经一次吸附和解吸后，CS和FS的含量分别从2.01%、1.67%提高到24.1%和22.4%，回收率分别为77.2%和68.5%。本工作优化的色谱过程是一个有前途的基础上，大规模制备CS和FS的进一步放大试验。(C)2008 Elsevier B. V.保留所有权利。
N-(p-Coumaroyl)serotonin (CS) and N-feruloylserotonin (FS) are two bioactive serotonin derivatives in safflower (Carthamus tinctorius L.) seeds with many biological effects. In the present study, the sorption and desorption characteristics of six widely used macroporous resins, coded D312, 860021, DM131, HZ801, AB8 and XDA-1, respectively, is critically evaluated and compared for enrichment and preparative separation of CS and FS. Static adsorption and desorption experiments on these resins showed that XDA-1 had the best adsorption and desorption equilibrium for CS and FS, and its adsorption equilibrium fits the best to the Langmuir isotherm. Dynamic adsorption and desorption experiments on XDA-1 resin packed column were conducted to establish the optimum parameters as: CS and FS concentration in sample solution 0.346 and 0.276 mg/mL, respectively, sample size 5-bed volumes (BV), sample flow rate 2 mL/min, temperature 25 degrees C (for adsorption); eluent 80% ethanol, 11 BV, flow rate 1 mL/min (for desorption). After one run of adsorption and desorption, the contents of CS and FS were increased from 2.01%, 1.67% to 24.1% and 22.4%, and the recoveries were 77.2% and 68.5%, respectively. The chromatographic process optimized in the present work is a promising basis for large scale preparation of CS and FS upon further scaling up tests. (C) 2008 Elsevier B.V. All rights reserved.