Epigenetic influence of KAT6B and HDAC4 in the development of skeletal malocclusion.
Epigenetic influence of KAT6B and HDAC4 in the development of skeletal malocclusion.
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DOI：
10.1016/j.ajodo.2013.06.016
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发表时间：
2013-10
影响因子：
3
通讯作者：
Sciote, James J.
中科院分区：
文献类型：
--
作者：
Huh, Ahrin;Horton, Michael J.;Cuenco, Karen T.;Raoul, Gwenael;Rowlerson, Anthea M.;Ferri, Joel;Sciote, James J.
遗传因素对错牙合畸形的影响包括对咀嚼肌和颌骨形态的遗传影响。然而，除了遗传变异之外，肌肉和骨骼的特征也受到表观遗传机制的影响，这些机制产生基因表达的差异。我们研究了已知通过组蛋白修饰改变基因表达的2种酶，即染色质修饰组蛋白乙酰转移酶KAT 6B和脱乙酰酶HDAC 4，以确定它们与颌骨变形咬合不正中肌肉骨骼变化的相关性。咬肌样本来自正颌外科手术的受试者，根据骨骼矢状和垂直发育不良分为6类错牙合。肌肉的特点是纤维类型的特性，通过免疫组化，和他们的总RNA被分离的基因表达的研究，通过微阵列分析和定量实时聚合酶链反应。肌球蛋白和收缩调节蛋白的快速亚型和KAT6B和HDAC4的基因表达是几倍大的咬肌从一个病人的深咬与一个开咬相比，与运动和活动相关的基因没有显着差异。在总人群中，HDAC 4（P = 0.03）和KAT 6B（P = 0.004）的表达在矢状面III类错患者中显著高于II类错患者，而HDAC 4倾向于与慢肌球蛋白I型呈负相关，与快肌球蛋白基因呈正相关，尤其是IIX型。这些数据支持了其他发表的关于骨骼肌纤维表型和骨生长的表观遗传调控的报告。需要进一步的调查，以阐明这种监管模式可能适用于肌肉骨骼发育和错牙合畸形。
Genetic influences on the development of malocclusion include heritable effects on both masticatory muscles and jaw skeletal morphology. Beyond genetic variations, however, the characteristics of muscle and bone are also influenced by epigenetic mechanisms that produce differences in gene expression. We studied 2 enzymes known to change gene expressions through histone modifications, chromatin-modifying histone acetyltransferase KAT6B and deacetylase HDAC4, to determine their associations with musculoskeletal variations in jaw deformation malocclusions. Samples of masseter muscle were obtained from subjects undergoing orthognathic surgery from 6 malocclusion classes based on skeletal sagittal and vertical dysplasia. The muscles were characterized for fiber type properties by immunohistochemistry, and their total RNA was isolated for gene expression studies by microarray analysis and quantitative real-time polymerase chain reaction. Gene expressions for fast isoforms of myosins and contractile regulatory proteins and for KAT6B and HDAC4 were severalfold greater in masseter muscles from a patient with a deepbite compared with one with an open bite, and genes related to exercise and activity did not differ substantially. In the total population, expressions of HDAC4 (P = 0.03) and KAT6B (P = 0.004) were significantly greater in subjects with sagittal Class III than in Class II malocclusion, whereas HDAC4 tended to correlate negatively with slow myosin type I and positively with fast myosin gene, especially type IIX. These data support other published reports of epigenetic regulation in the determination of skeletal muscle fiber phenotypes and bone growth. Further investigations are needed to elucidate how this regulatory model might apply to musculoskeletal development and malocclusion.
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影响因子：
--
作者：
Ackermann MA;Kontrogianni-Konstantopoulos A
通讯作者：
Kontrogianni-Konstantopoulos A
影响因子：
4.8
作者：
Mahoney, DJ;Parise, G;Tarnopolsky, MA
通讯作者：
Tarnopolsky, MA
影响因子：
8
作者：
Pelletier, N;Champagne, N;Yang, XJ
通讯作者：
Yang, XJ
影响因子：
2.6
作者：
Delaire, J
通讯作者：
Delaire, J
影响因子：
5.5
作者：
Pandorf, Clay E.;Haddad, Fadia;Baldwin, Kenneth M.
通讯作者：
Baldwin, Kenneth M.