Effect of pre-germination at varying stages of embryonic growth length on chemical composition and protein profile of Thai rice (Oryza sativa L.)
Effect of pre-germination at varying stages of embryonic growth length on chemical composition and protein profile of Thai rice (Oryza sativa L.)
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DOI：
10.1016/j.anres.2018.05.009
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发表时间：
2018-02
影响因子：
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通讯作者：
W. Kupkanchanakul;M. Kadowaki;M. Kubota;O. Naivikul
中科院分区：
文献类型：
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作者：
W. Kupkanchanakul;M. Kadowaki;M. Kubota;O. Naivikul
在不同阶段的胚胎生长长度（EGL）（0.5-7毫米，为30-56小时的预发芽时间）的化学组成和蛋白质分布的影响进行了研究，在三个不同的泰国水稻品种-SPT 1（糯），PTT 1（低直链淀粉），和PNL 2（高直链淀粉）。在0.5 ~ 1 mm长的发芽层（第一阶段）预发芽，除PNL 2的粗蛋白含量和SPT 1的粗脂肪含量分别在3-7 mm长的发芽层（发芽阶段）和2-3 mm长的发芽层（第三阶段）发生显著变化外，其余水稻品种的总淀粉、粗蛋白和粗脂肪含量均发生显著变化。蛋白质图谱分析表明，谷蛋白分子以二硫键连接的亚基形式存在。预萌发到SPT 1和PTT 1的第二阶段以及PNL 2的第一阶段导致谷蛋白原（proglutelin，51.6kDa）裂解为酸性谷蛋白（acidic-glutelin，31.9kDa）和碱性谷蛋白（basic-glutelin，17.8kDa），并且还增加了13 kDa醇溶蛋白的相对浓度。在30 °C下预发芽32-48 h，这三个水稻品种的蛋白质的生物修饰功能，特别是谷蛋白二硫键的还原功能明显。
The effect of pre-germination at varying stages of embryonic growth length (EGL) (0.5–7 mm, for 30–56 h of pre-germination time) on the chemical composition and protein profiles were investigated in three different Thai rice cultivars—SPT1 (waxy), PTT1 (low amylose), and PNL2 (high amylose). Pre-germination at 0.5–1 mm of EGL (first-stage) of all rice led to a significant change in most of the total starch, crude protein, and crude fat contents except in the crude protein content of PNL2 and the crude fat content of SPT1, which significantly changed at 3–7 mm of EGL (malted-stage) and at 2–3 mm of EGL (third-stage), respectively. Analysis of protein profiles from all rice samples indicated that glutelin molecules existed in subunits linked by disulfide bonds. Pre-germination to the second-stage of SPT1 and PTT1, and to the first-stage of PNL2 caused the cleavage of proglutelin (∼51.6 kDa) into acidic-glutelin (∼31.9 kDa) and basic-glutelin (∼17.8 kDa) and also increased the relative concentration of 13 kDa prolamins. Pre-germination of these three rice cultivars at 30 °C for 32–48 h clearly indicated the function as bio-modification of rice proteins, particularly in the reduction of glutelin disulfide bonds.