Light‐Induced CREB Phosphorylation and Gene Expression in Rat Retinal Cells
Light‐Induced CREB Phosphorylation and Gene Expression in Rat Retinal Cells
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大鼠视网膜细胞光诱导 CREB 磷酸化和基因表达
DOI：
10.1046/j.1471-4159.1995.65041499.x
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发表时间：
1995
影响因子：
4.7
通讯作者：
M. Hagiwara
中科院分区：
文献类型：
--
作者：
Kazuhiko Yoshida;J. Imaki;H. Matsuda;M. Hagiwara
翻译后摘要：光诱导表达的c‐fos和神经肽生长抑素（SS）在大鼠视网膜细胞的信号通路进行了研究。闪烁光诱导内核层和神经节细胞层中的c-fos和SS mRNA。由于c-fos和SS基因在其启动子中均具有环AMP反应元件（CRE），因此用磷酸化CREB特异性抗体检测视网膜细胞中的CRE结合蛋白（CREB）磷酸化。闪光灯和给予L-型Ca 2+通道激活剂Bay K 8644均可诱导表达c-fos/SS mRNA的无长突细胞和神经节细胞核中CREB的磷酸化。在Bay K 8644给药后，这些细胞可以用抗钙调蛋白激酶II（抗CaM激酶II）单克隆抗体和磷酸化CREB特异性多克隆抗血清进行双重染色，表明磷酸化CREB在Ser 133和CaM激酶II处在神经视网膜中共定位。
Abstract: The signal pathway for light‐induced expression of c‐fos and the neuropeptide somatostatin (SS) in rat retinal cells was investigated. Flashing light induced c‐fos and SS mRNA in the inner nuclear layer and the ganglion cell layer. As both c‐fos and SS genes have a cyclic AMP response element (CRE) in their promoters, CRE‐binding protein (CREB) phosphorylation in retinal cells was examined with a phospho‐CREB‐specific antibody. Both flashing light and administration of the L‐type Ca2+ channel activator Bay K 8644 induced phosphorylation of CREB in the nuclei of the amacrine cells and the ganglion cells where c‐fos/SS mRNAs were expressed. These cells could be double‐stained with anti‐calmodulin kinase II (anti‐CaM kinase II) monoclonal antibody and phospho‐CREB‐specific polyclonal antiserum after Bay K 8644 administration, indicating the colocalization of phosphorylated CREB at Ser133 and CaM kinase II in the neural retina.
DOI：
10.1073/pnas.83.18.6682
发表时间：
1986-09-01
影响因子：
11.1
作者：
MONTMINY, MR;SEVARINO, KA;GOODMAN, RH
通讯作者：
GOODMAN, RH
Ca2/钙调蛋白依赖性蛋白激酶 IV 的表征。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Enslen,H;Sun,P;Brickey,D;Soderling,SH;Klamo,E;Soderling,TR
通讯作者：
Soderling,TR
影响因子：
56.9
作者：
SHENG, M;THOMPSON, MA;GREENBERG, ME
通讯作者：
GREENBERG, ME