Biophysical Regulation of Cell Behavior-Cross Talk between Substrate Stiffness and Nanotopography.
Biophysical Regulation of Cell Behavior-Cross Talk between Substrate Stiffness and Nanotopography.
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DOI：
10.1016/j.eng.2017.01.014
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发表时间：
2017-03
期刊：
影响因子：
--
通讯作者：
Leong KW
中科院分区：
文献类型：
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作者：
Yang Y;Wang K;Gu X;Leong KW
细胞外基质（ECM）的硬度和纳米形貌特征影响体内许多发育、生理和病理过程。因此，这些生物物理线索已被应用于调节细胞行为的几乎所有方面，从细胞粘附和扩散到增殖和分化。细胞行为的生物物理调节的描述对于新生物材料、植入物和医疗设备的合理设计至关重要。之前已经分别回顾了硬度和地形线索对细胞行为的影响；然而，尽管表型表现相似，但刚度和纳米形貌线索对细胞行为的交织影响尚未得到很好的描述。在这里，我们首先回顾了基底刚度和纳米形貌对细胞行为的影响，然后重点关注从整合素到细胞核的生物物理信号的细胞内传递。人们尝试将细胞行为的细胞外调节与生物物理线索联系起来。然后，我们讨论剖析细胞行为的生物物理调节以及将对这些线索的机制理解转化为组织工程和再生医学的挑战。
The stiffness and nanotopographical characteristics of the extracellular matrix (ECM) influence numerous developmental, physiological, and pathological processes in vivo. These biophysical cues have therefore been applied to modulate almost all aspects of cell behavior, from cell adhesion and spreading to proliferation and differentiation. Delineation of the biophysical modulation of cell behavior is critical to the rational design of new biomaterials, implants, and medical devices. The effects of stiffness and topographical cues on cell behavior have previously been reviewed, respectively; however, the interwoven effects of stiffness and nanotopographical cues on cell behavior have not been well described, despite similarities in phenotypic manifestations. Herein, we first review the effects of substrate stiffness and nanotopography on cell behavior, and then focus on intracellular transmission of the biophysical signals from integrins to nucleus. Attempts are made to connect extracellular regulation of cell behavior with the biophysical cues. We then discuss the challenges in dissecting the biophysical regulation of cell behavior and in translating the mechanistic understanding of these cues to tissue engineering and regenerative medicine.