Single-Cell Joint Profiling of Open Chromatin and Transcriptome by Paired-Seq.
Single-Cell Joint Profiling of Open Chromatin and Transcriptome by Paired-Seq.
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通过配对序列对开放染色质和转录组进行单细胞联合分析。
DOI：
10.1007/978-1-0716-2899-7_10
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Ren,Bing
中科院分区：
文献类型：
--
作者：
Zhu,Chenxu;Wang,Zhaoning;Ren,Bing
同时检测同一细胞的染色质可及性和转录，有望极大地促进复杂组织中细胞类型特异性基因调控程序的解剖。Paired-seq能够并行分析多达一百万个细胞的开放染色质和核转录组。它通过使用组合条形码化策略实现超高通量单细胞多组学，所述组合条形码化策略涉及多重DNA条形码与染色质DNA片段和逆转录产物的顺序连接，然后对所得DNA文库进行高通量DNA测序，并基于细胞特异性条形码对单细胞多组学图谱进行去卷积。
Simultaneous detection of chromatin accessibility and transcription from the same cells promises to greatly facilitate the dissection of cell-type-specific gene regulatory programs in complex tissues. Paired-seq enables joint analysis of open chromatin and nuclear transcriptome from up to a million cells in parallel. It achieves ultra-high-throughput single-cell multiomics with the use of a combinatorial barcoding strategy involving sequential ligation of multiplexed DNA barcodes to chromatin DNA fragments and reverse transcription products, followed by high-throughput DNA sequencing of the resulting DNA libraries and deconvolution of single-cell multiomic maps based on cell-specific barcodes.