Control of pH in a System of Flowing Solution Culture using a Microcomputer
Control of pH in a System of Flowing Solution Culture using a Microcomputer
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微机流动溶液培养系统pH值控制
DOI：
10.1093/jxb/35.12.1860
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
R. Canaway
中科院分区：
文献类型：
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作者：
D. Hatch;R. Canaway
本文介绍了一种用微型计算机控制流水培养pH的系统，并简要介绍了该系统在多年生黑麦草(Lolium perenne L.)植株生长在pH分别为5-0、5-5、60和7-0的营养液中。关键词：PH值；微型计算机。植物根部周围溶液pH值的变化会显著影响营养离子的吸收速度，因此需要对溶液培养技术进行确定性研究，因此需要精确控制pH值。已经设计了几个系统来研究流动溶液培养中完整植物对离子的吸收，在流动溶液培养中，通过自动监测和控制(Asher，Ozanne和Loneragan，1965；Clement，Hopper，Canaway和Jones，1974)或人工控制(Reisenaur，1968；Bhat，1980)，保持营养离子的pH值和浓度接近恒定。然而，对于手动控制系统，两次采样之间的pH值可能会有相当大的变化。Asher等人对pH的自动控制。(1965)和克莱门特等人。(1974)是基于自动滴定仪控制器，灵活性有限。Glass、Siddiqi和Deane-Drummond(1983)已经证明了微型计算机的多功能性，用于测量完整植物在15分钟内耗尽搅拌营养液期间的离子活度。现在我们描述微型计算机在克莱门特等人的系统中的监测和控制的应用。(1974)在较长时期内提供灵活和精确的pH控制，并简要介绍了使用多年生黑麦草(Lolium Perenne L.)的系统性能。作为实验植物。组成和操作从最初的流动溶液培养系统(Clement et al.，1974)对培养单元的改进如图1所示。四个培养单元中的每一个都配备了一个含有电极的流动池，用于连续采样和监测循环营养液的pH。一种基于微计算机3的控制组件，其通信地址应该是该微计算机3。此内容从UE，2016 10月18日04：48：54 UTC从157.55.39.56下载，所有使用均受http://about.jstor.org/terms Hatch和Canaway-Automatic pH Control 1861的限制，通过自动输送酸或碱将每个培养单元中的溶液的pH维持在规定值。营养液中的钾浓度由人工监测，每天调整两次；其他营养素按钾的比例供应。自动控制营养液温度的设备是记录仪-3控制组件
A system is described which uses a microcomputer to control pH in flowing solution culture and a brief account is given of its performance when intact perennial ryegrass (Lolium perenne L.) plants were grown in nutrient solutions with pH controlled at 5-0, 5-5, 6 0 and 7-0. Key words: pH; Microcomputer. INTRODUCTION Changes in pH of the solution surrounding plant roots can markedly affect the rate of uptake of nutrient ions and definitive studies involving solution culture techniques, therefore, require precise control of pH. Several systems have been devised for studying ion uptake by intact plants in flowing solution culture in which the pH and the concentration of nutrient ions are held near constant either by automatic monitoring and control (Asher, Ozanne, and Loneragan, 1965; Clement, Hopper, Canaway, and Jones, 1974) or manual control (Reisenaur, 1968; Bhat, 1980). With manual control systems, however, there may be considerable changes in pH between sampling. The automatic control of pH by Asher et al. (1965) and Clement et al. (1974) is based on auto-titrator controllers, with limited flexibility. Glass, Siddiqi, and Deane-Drummond (1983) have demonstrated the versatility of micro computers for measuring ion activities during depletion of stirred nutrient solutions by intact plants over a period of 15 min. We now describe the application of a microcomputer for monitoring and control in the system of Clement et al. (1974) to give flexible and precise pH control over extended periods, together with a brief account of the performance of the system using perennial ryegrass (Lolium perenne L.) as the experimental plant. COMPONENTS AND OPERATION The modification to a culture unit from the original system of flowing solution culture (Clement et al., 1974) is shown diagrammatically in Fig. 1. Each of four culture units is equipped with a flow cell containing an electrode for continuous sampling and monitoring of pH of the circulating nutrient solution. A control assembly based on a microcomputer 3 To whom correspondence should be addressed. This content downloaded from 157.55.39.56 on Tue, 18 Oct 2016 04:48:54 UTC All use subject to http://about.jstor.org/terms Hatch and Canaway—Automatic pH Control 1861 maintains pH of the solution in each culture unit at prescribed values by automatic delivery of acid or alkali. The concentration of potassium in the nutrient solution is monitored manually and adjusted twice daily; other nutrients are supplied in proportion to potassium. Equipment for automatically controlling the temperature of the nutrient solution is Recorder —<3 Control Assembly