Clumps of mesenchymal stem cells/extracellular matrix complexes directly reconstruct the functional periodontal tissue in a rat periodontal defect model
Clumps of mesenchymal stem cells/extracellular matrix complexes directly reconstruct the functional periodontal tissue in a rat periodontal defect model
复制标题
DOI：
10.1002/term.3343
复制
发表时间：
2022-08
影响因子：
3.3
通讯作者：
Hisakatsu Sone;M. Kajiya;K. Takeda;Shinya Sasaki;Susumu Horikoshi;Souta Motoike;Shin Morimoto;Hiroki Yoshii;Mai Yoshino;T. Iwata;K. Ouhara;S. Matsuda;N. Mizuno
中科院分区：
文献类型：
--
作者：
Hisakatsu Sone;M. Kajiya;K. Takeda;Shinya Sasaki;Susumu Horikoshi;Souta Motoike;Shin Morimoto;Hiroki Yoshii;Mai Yoshino;T. Iwata;K. Ouhara;S. Matsuda;N. Mizuno
牙周炎是一种炎症性疾病，其特征是牙齿支持的牙周组织破坏，包括牙骨质、牙周膜和牙槽骨。为了再生受损的牙周组织，间充质干细胞（MSC）引起了科学和医学的广泛关注。最近，我们生成了 MSCs/细胞外基质 (ECM) 复合物 (C-MSCs) 团块，它由细胞和自身产生的 ECM 组成。 C-MSCs无需人工支架即可移植到病变区域以诱导组织再生。为了利用 C-MSC 开发可靠的无支架牙周组织再生细胞疗法，本研究研究了 C-MSC 的牙周组织再生能力和移植细胞的行为。从大鼠股骨中分离出大鼠骨髓来源的 MSC。使用微量移液器尖端划伤汇合的细胞，然后撕下。将片材卷起以形成三维圆形细胞团，即 C-MSC。然后，将 10 个 C-MSC 移植到大鼠牙周开窗缺损模型中。为了追踪缺损处的移植细胞，还使用了 PKH26 标记的细胞。显微 CT 和组织学分析表明，C-MSC 移植在大鼠牙周缺损模型中诱导成功的牙周组织再生。有趣的是，重建组织中的大多数细胞，包括牙骨质、牙周膜和牙槽骨，都是PKH26阳性供体细胞，表明MSCs直接形成组织。这项研究展示了一种利用 C-MSC 治疗牙周病的有前途的无支架 MSC 移植策略，并提供了 MSC 多能性诱导成功牙周组织再生的意义。
Periodontitis is an inflammatory disease characterized by tooth‐supporting periodontal tissue destruction, including the cementum, periodontal ligament, and alveolar bone. To regenerate the damaged periodontal tissue, mesenchymal stem cells (MSCs) have attracted much scientific and medical attention. Recently, we generated clumps of MSCs/extracellular matrix (ECM) complexes (C‐MSCs), which consist of cells and self‐produced ECM. C‐MSCs can be transplanted into lesion areas without artificial scaffold to induce tissue regeneration. To develop reliable scaffold‐free periodontal tissue regenerative cell therapy by C‐MSCs, this study investigated the periodontal tissue regenerative capacity of C‐MSCs and the behavior of the transplanted cells. Rat bone marrow‐derived MSCs were isolated from rat femur. Confluent cells were scratched using a micropipette tip and then torn off. The sheet was rolled to make a three‐dimensional round clump of cells, C‐MSCs. Then, ten C‐MSCs were grafted into a rat periodontal fenestration defect model. To trace the grafted cells in the defect, PKH26‐labeled cells were also employed. Micro‐CT and histological analyses demonstrated that transplantation of C‐MSCs induced successful periodontal tissue regeneration in the rat periodontal defect model. Interestingly, the majority of the cells in the reconstructed tissue, including cementum, periodontal ligaments, and alveolar bone, were PKH26 positive donor cells, suggesting the direct tissue formation by MSCs. This study demonstrates a promising scaffold‐free MSCs transplantation strategy for periodontal disease using C‐MSCs and offers the significance of multipotency of MSCs to induce successful periodontal tissue regeneration.