Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers
Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers
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DOI：
10.1007/s001220050867
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发表时间：
1998-07-01
影响因子：
5.4
通讯作者：
King, GJ
中科院分区：
文献类型：
--
作者：
Maliepaard, C;Alston, FH;King, GJ
利用RFLP、RAPD、同工酶、AFLP、SCAR和微卫星标记，以152个苹果品种的Prima × Fiesta后代为材料，构建了Prima和Fiesta的连锁图谱。每个亲本共获得17个连锁群，它们对应于17条苹果单倍体染色体。这些地图使用67个多等位基因标记进行比对，这些标记在双亲中都是杂合的。大量重复的RFLP位点被观察到，在一些情况下，连锁RFLP标记在一个连锁群显示相应的连锁在另一个连锁群。结果表明，苹果果实酸度基因Ma和自交不亲和基因S的抗赤霉病基因和抗玫瑰曲叶蚜基因分别位于Vf和Sd（1）的图谱上。该图谱标记密度高，共显性RFLP和微卫星标记数量多，是一个理想的参考图谱，可用于其它后代的遗传分析，也是一个有价值的数量性状基因座定位工具。
Linkage maps for the apple cultivars 'Prima' and 'Fiesta' were constructed using RFLP, RAPD, isozyme, AFLP, SCAR and microsatellite markers in a 'Prima' x 'Fiesta' progeny of 152 individuals. Seventeen linkage groups, putatively corresponding to the seventeen haploid apple chromosomes, were obtained for each parent. These maps were aligned using 67 multi-allelic markers that were heterozygous in both parents. A large number of duplicate RFLP loci was observed and, in several instances, linked RFLP markers in one linkage group showed corresponding linkage in another linkage group. Distorted segregation was observed mainly in two regions of the genome, especially in the male parent alleles, Map positions were provided for resistance genes to scab and rosy leaf curling aphid (Vf and Sd(1), respectively) for the fruit acidity gene Ma and for the self-incompatibility locus S. The high marker density and large number of mapped codominant RFLPs and some microsatellite markers make this map an ideal reference map for use in other progenies also and a valuable tool for the mapping of quantitative trait loci.